Abstract
Lepomis microlophus (redear sunfish) were collected from Martin Lake, an east Texas reservoir, as well as a reference lake 8 km upstream in the same drainage system. Martin Lake received aqueous selenium-laden effluent from electrical generator plant systems used to collect fly ash, scrubber sludge, and bottom ash; the reference lake received no such effluent (Texas Parks and Wildlife Department, unpublished report). Neutron activation analysis was used to monitor selenium levels in the hepatopancreas (i.e., the liver and associated, disseminated exocrine pancreas), which is one of the major organs used in monitoring selenium exposure. Fish collected from areas adjacent to the selenium-discharge sites in Martin Lake accumulated about 19.8 ppm in the hepatopancreas, compared to approximately 8.4 ppm for fish collected further from the discharge site. Reference redear sunfish accumulated normal levels of 1.8 ppm in the hepatopancreas. Hematological abnormalities were most severe for fish collected from areas adjacent to the selenium discharge site. These abnormalities included altered leukocyte distribution with elevated numbers of hemoblasts, reduced hematocrits, lower hemoglobin concentrations, slightly reduced numbers of erythrocytes, 12% and 23% reductions in mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV), respectively, and microcytic, poikilocytic erythrocytes with elevated numbers of nuclear shadows. These data present a striking illustration of a case in which the hematological characteristics of a freshwater teleost were correlated with selenium accumulation in a critical organ following chronic selenium exposure. Hematological data provided a sensitive indicator of the severity of the impact of selenium-laden generator plant effluent on this teleost species.
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References
Best, R.: A hematological slide rule for calculating the corpuscular constants. J. Lab. Clin. Med.34, 434 (1949).
Chow, C. K., and C. J. Chen: Dietary selenium and age-related susceptibility of rat erythrocytes to oxidative damage. J. Nutr.110, 2460 (1980).
Cooper, J. A.: Factors determining the ultimate detection sensitivity of Ge-Li gamma-ray spectrometers. Battelle Memorial Northwest Laboratory, Richland, WA, BNWL-SA-2940 (1970).
Draize, J. H., and O. A. Beath: Observations on the pathology of blind staggers and alkali disease. J. Am. Vet. Med. Assoc.86, 753 (1935).
Ellis, M. M., H. L. Motley, M. D. Ellis, and R. O. Jones: Selenium poisoning in fishes. Proc. Soc. Exp. Biol. Med.36, 519 (1937).
Franke, K. W.: A new toxicant occurring naturally in certain samples of plant foodstuffs. I. Results obtained in preliminary feeding trials. J. Nutr.8, 597 (1934).
Franke, K. W., and V. R. Potter: A new toxicant occurring naturally in certain samples of plant foodstuffs. III. Hemoglobin levels observed in white rats which were fed toxic wheat. J. Nutr.8, 615 (1934).
—: A new toxicant occurring naturally in certain samples of plant foodstuffs. IX. Toxic effects of orally ingested selenium. J. Nutr.10, 213 (1935).
Hilton, J. W., P. V. Hodson, and S. J. Slinger: The requirement and toxicity of selenium in rainbow trout (Salmo gairdneri). J. Nutr.110, 2527 (1980).
Jacob, S. W., and C. A. Francone: Structure and function in man. Philadelphia: W. B. Saunders (1974).
Lillie, R. D., and M. I. Smith: Histogenesis of hepatic cirrhosis in chronic food selenosis. Am. J. Pathol.16, 223 (1940).
Lowerre, R. W.: Assistant Attorney General for the State of Texas, Reagan Building, 5th Floor, Austin, Texas 78711, (1978).
Mishra, S., and A. K. Srivastava: Hematology as index of sublethal toxicity of zinc in a freshwater teleost. Bull. Environ. Contam. Toxicol.22, 695 (1979).
Moxon, A. L., and M. Rhian: Selenium poisoning. Physiol. Rev.23, 305 (1943).
Neal, K. G.: Vitamins and minerals. Vol. 1, p. 16. Long Beach: Elot Publ. Co. (1977).
Poston, H. A., G. F. Combs, and L. Leibovitz: Vitamin E and selenium interrelations in the diet of Atlantic salmon: Gross histological and bio-chemical deficiency signs. J. Nutr.106, 892 (1976).
Smith, M. I., E. F. Stohlman, and R. D. Lillie: The toxicity and pathology of selenium. J. Pharmacol. Exptl. Therap.60, 449 (1937).
Sorensen, E. M. B., C. W. Harlan, J. S. Bell, T. L. Bauer, and A. Pradzynski: Hepatocyte changes following selenium accumulation in a freshwater teleost. Amer. J. Forensic Med. Pathol.3, 1 (1982a).
Sorensen E. M. B., C. W. Harlan, and J. S. Bell: Renal changes in selenium-exposed fish. Amer. J. Forensic Med. Pathol.3 (1982b).
Srivastava, A. K., and S. Mishra: Blood dyscrasia in a teleost fish,Colisa fasciatus, associated with cadmium poisoning. J. Comp. Pathol.89, 609 (1979).
Yuki, R.: Blood cell constituents in fish. IV. On the “nuclear shadow” found in blood smear preparations. Bull. Jap. Soc. Sci.Fish.26, 490 (1960).
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Sorensen, E.M.B., Bauer, T.L. Hematological dyscrasia in teleosts chronically exposed to selenium-laden effluent. Arch. Environ. Contam. Toxicol. 12, 135–141 (1983). https://doi.org/10.1007/BF01059571
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DOI: https://doi.org/10.1007/BF01059571