Abstract
Methods for the screening of fungal cultures for toxic secondary metabolites are reviewed. Thin layer Chromatography (TLC) and high-performance liquid Chromatography (HPLC) are good general analytical methods for secondary metabolites in unpurified extracts. The combination of normal phase TLC using different chemical spray reagents with reversed phase HPLC, using alkylphenone retention indices and diode array detection, is a powerful technique for identifying the individual mycotoxins detected. The results of the screening methods are very dependent of the growth media and incubation conditions and a general method for the detection ofPenicillium, Aspergillus, Fusarium, Alternaria andCladosporium toxins is suggested.
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References
Barr JG, Dawney GA (1975) A multiple inoculation technique for the screening of fungal isolates for the evaluation of growth and mycotoxin production on agar substrates. J Sci Fd Agric 26:1561–1566
Beaver RW (1986) Gas chromatography in mycotoxin analysis. See Cole RJ (1986), pp 265–292
Betina V (ed) (1984) Mycotoxins Production, Isolation, Separation and Purification. Developments in Food Science 8. Elsevier, Amsterdam, 528 pp
Bullerman LB (1974) A screening medium and method to detect several mycotoxins in mold cultures. J Milk Food Technol 37:1–3
Chang TT, Lay JO, Francel RJ (1984) Direct analysis of thin-layer chromatography spots by fast atom bombardment mass spectrometry. Anal Chem 56:109–111
Cole RJ (ed) (1986) Modern methods in the analysis and structural elucidation of mycotoxins. Academic Press, Orlando, 471 pp
Cole RJ, Cox RH (1981) Handbook of toxic fungal metabolites. Academic Press, New York, 874 pp
Duracková Z, Betina V, Nemec P (1976) Systematic analysis of mycotoxins by thin-layer chromatography. J Chromatogr 116:141–154
Filtenborg O, Frisvad JC (1980) A simple screening method for toxigenic moulds in pure cultures. Lebensm Wiss Technol 13:128–130
Filtenborg O, Frisvad JC, Svendsen JA (1983) Simple screening method for molds producing intracellular mycotoxins in pure cultures. Appl Environ Microbiol 45:581–585
Frisvad JC (1986) Taxonomic approaches to mycotoxin identification. See Cole RJ (1986), pp 415–457
— (1987) High-performance liquid chromatographic determination of profiles of mycotoxins and other secondary metabolites. J Chromatogr 392:333–347
Frisvad JC, Filtenborg O (1983) Classification of terverticillate peniciliia based on profiles of mycotoxins and other secondary metabolites. Appl Environ Microbiol 46:1301–1310
Frisvad JC, Thrane U (1987) Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode array detection). J Chromatogr 404:195–214
Ghosh AC, Manmade A, Bousquet JM, Townsend JM, Demain AL (1978) A convenient thin-layer chromatographic method for the detection of cyclochlorotine and simatoxin, toxins fromPenicillium islandicum Sopp. Experientia 34:819–820
Gorst-Allman CP, Steyn PS (1979) Screening methods for the detection of thirteen common mycotoxins. J Chromatogr 175:325–331
- (1984) Applications of chromatographic techniques in the separation, purification and characterization of mycotoxins. See Betina (1984), pp 59–85
Harwig J, Scott PM (1971) Brine shrimp (Artemia salina L.) larvae as a screening system for fungal toxins. Appl Microbiol 21:1101–1106
Krogh P (ed) (1987) Mycotoxins in food. Academic Press, New York, 280 pp
Leistner L, Eckardt C (1979) Vorkommen toxinogener Penicillien bei Fleisch Erzeugnisse. Fleischwirtschaft 59:1892–1896
Lötzsch R, Tauchmann F, Meyer W (1974) Einsatz des “Stomacher” in der Mykotoxin-Analytik. Fleischwirtschaft 54:943–945
Moskowitz GJ, Cayle T (1974) a method for the detection of 3-nitropropionic acid in crude biological extracts. Cereal Chem 51:96–105
Paterson RRM (1986) Standardized one- and two-dimensional thin-layer chromatographic methods for the identification of secondary metabolites inPenicillium and other fungi. J Chromatogr 368:249–264
Paterson RRM, Simmonds MSJ, Blaney WM (1987) Mycopesticidal effects of characterized extracts ofPenicillium isolates and purified secondary metabolites (including mycotoxins) onDrosophila melanogaster andSpodoptora littoralis. J Invert Pathol 50:124–133
Plattner RD (1986) Mass spectrometry-mass spectrometry as a tool for mycotoxin analysis. See Cole (1986), pp 393–414
Scott PM (1981) High performance liquid chromatographic determination of mycotoxins. In: Lawrence JF (ed) Trace analysis, Academic Press, New York, pp 193–266
Scott PM, Kennedy BPC (1973) Improved method for the thin-layer chromatographic determination of patulin in apple juice. J Assoc Offic Anal Chem 56:813–816
Scott PM, Lawrence JW, Walbeek W van (1970) Detection of mycotoxins by thin-layer chromatography: Application to screening of fungal extracts. Appl Microbiol 20:839–842
Shepherd MJ (1986) High performance liquid chromatography and its application to the analysis of mycotoxins. See Cole (1986), pp 293–333
Steyn PS (1969) The separation and detection of several mycotoxins by thin-layer chromatography. J Chromatogr 45:473–475
Thrane U (1986) Detection of toxigenicFusarium isolates by thin-layer chromatography. Lett Appl Microbiol 3:93–96
Vesonder RF, Rohwedder WK (1986) Gas chromatographicmass spectrometric analysis of mycotoxins. See Cole (1986), pp 335–351
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Frisvad, J.C., Filtenborg, O. & Thrane, U. Analysis and screening for mycotoxins and other secondary metabolites in fungal cultures by thin-layer chromatography and high-performance liquid chromatography. Arch. Environ. Contam. Toxicol. 18, 331–335 (1989). https://doi.org/10.1007/BF01062357
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DOI: https://doi.org/10.1007/BF01062357