Abstract
Plasma pharmacokinetics of sodium fluorescein, fluorescein isothiocyanate conjugated bovine serum albumin, and a graded series of dextrans of 19,400 to 71,800 MW were monitored continuously using a noninvasive photometric technique in individual blood vessels of tissue grown in a rabbit ear chamber. The data obtained were fitted with a two-compartment open model to obtain an effective permeability and an effective clearance. Both parameters decreased with increasing molecular radius for dextrans. Values for albumin were considerably less than expected on the basis of molecular radius, presumably due to the configuration, charge, and binding characteristics of albumin.
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Supported by grants from the National Science Foundation, the R. K. Mellon Foundation, and the American Cancer Society. In addition, R. K. Jain is a recipient of a Research Career Development Award from the National Institutes of Health, and a Guggenheim Fellowship.
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Nugent, L.J., Jain, R.K. Two-compartment model for plasma pharmacokinetics in individual blood vessels. Journal of Pharmacokinetics and Biopharmaceutics 12, 451–461 (1984). https://doi.org/10.1007/BF01062668
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DOI: https://doi.org/10.1007/BF01062668