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Alkaloid formation by Catharanthus roseus cells in a packed column biofilm reactor

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Summary

Catharanthus roseus cells producing indole alkaloids were grown on surfaces of Ca-alginate beads within the interspacial volume of a packed column. Production media was circulated through the packed column in an upflow mode. Growth and indole alkaloid formation were quantified and compared with suspension culture of cells. Final alkaloid concentration and alkaloid yield obtained in the packed bed was superior to those obtained in suspension culture. This is thought to be due to improved cell-cell contact and interaction in the packed column.

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References

  1. F. Kargi and M. Z. Rosenberg,Biotechnol. Progress 1987.3(1):108.

    Google Scholar 

  2. O. P. Sahai and M. Knuth,Biotechnol. Progress 1985.1:1–4.

    Google Scholar 

  3. M. W. Fowler and G. S. Sarkissian. 1983. In “Advances in Biotechnological Processes” Eds. A. Mizrahi and A. Van Wezel. Vol.2 pp. 135–158. Alan R. Liss, NY.

    Google Scholar 

  4. D. Drapeau, H. W. blanch and C. R. Wilke. 1987.Biotechnol. Bioeng. 30:946–953.

    Google Scholar 

  5. M. W. Fowler. 1983. In “Plant Biotechnology” Eds. S. H. Mantel and H. Smith. Cambridge University Press pp. 3–37.

  6. M. Curtin.Bio/Technology October 1983. Vol. 1 pp. 649–657.

    Google Scholar 

  7. O. P. Sahai and M. L. Shuler 1984.Biotechnol. Bioeng. 26:27–36.

    Google Scholar 

  8. O. P. Sahai and M. L. Shuler 1984.Biotechnol. Bioeng. 26:111–120.

    Google Scholar 

  9. M. L. Shuler, O. P. Sahai and G. A. Hallsby 1982.Annals N.Y. Acad. Sci. 413:373–382.

    Google Scholar 

  10. P. Brodelius and K. Mosbach 1982.J. Chem. Technol. Biotechnol. 22:330–337.

    Google Scholar 

  11. P. Brodelius and K. Mosbach 1982.Adv. in Applied Microbial. 28:1–26.

    Google Scholar 

  12. H. R. Felix and K. Mosbach 1982.Biotechnol. Letters 4(3):181–186.

    Google Scholar 

  13. L. Linse and P. Brodelius 1982.Annals N.Y. Acad. Sci. 487–490.

  14. I. Freidel 1987. M. S. Thesis, Washington University. St. Louis MO.

  15. R. A. Dixon 1985. Isolation and Maintenance of Callus and Cell Suspension Cultures. In “Plant Cell Culture: A Practical Approach”, Ed. R. A. Dixon. IRL Press, Washington, D.C.

    Google Scholar 

  16. Glucose Diagnositc Kit No. 115. 1986. Sigma Chemical Company. St. Louis MO.

  17. S. L. Lee and A. I. Scott 1981.Phytochemistry 20(8):1841–1843.

    Google Scholar 

  18. L. E. Towill and P. Mazur 1975.Can. J. Botany 53:1097–1102.

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Engineering Laboratory Department of Chemical Engineering, Washington Universit, 63130, St. Louis, Missouri

    Fikret Kargi

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  1. Fikret Kargi
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Kargi, F. Alkaloid formation by Catharanthus roseus cells in a packed column biofilm reactor. Biotechnol Lett 10, 181–186 (1988). https://doi.org/10.1007/BF01134823

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  • DOI: https://doi.org/10.1007/BF01134823

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