Summary
A significant fraction (10–40%) of the indole alkaloids produced byCatharanthus roseus was observed to be secreted into the medium. When a neutral polymeric resin, known to adsorb these alkaloids, was added to the cultivation medium, the accumulation of total indole alkaloids and the specific alkaloids, ajmalicine and serpentine were stimulated. Sorbent addition was also observed to result in increased ratios of ajmalicine to serpentine, which suggests the potential of using in situ adsorption to direct metabolism toward a specific product or intermediate within a given pathway.
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Payne, G.F., Payne, N.N., Shuler, M.L. et al. In situ adsorption for enhanced alkaloid production byCatharanthus roseus . Biotechnol Lett 10, 187–192 (1988). https://doi.org/10.1007/BF01134827
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DOI: https://doi.org/10.1007/BF01134827