Summary
Choroid plexuses from early and late sheep foetuses were examined by an improved freeze-fracture technique and the use of double-replicas to define the structure of the tight junction network of the epithelial cells. ‘Complex’ strands which consist of two normal parallel strands separated by a single row of pits or particles are defined and demonstrated in complementary faces. Since this strand variety was encountered in the same proportion in the different developmental stages investigated, it could not be correlated with changes in permeability. It is more likely that the ‘complex’ strands are associated with the transfer of gap junction particles within the membrane. The question of the significance of discontinuities in P face ridges was also resolved by the double replica technique: the few discontinuities which were observed could be accounted for by particles in the complementary E faces. Furthermore, approximately the same number of such junction displacements was found in early and late stages which makes it unlikely that this phenomenon could contribute to changes in permeability. Thus it has not been possible so far to relate any structural feature of the tight junction network in developing choroid plexus epithelial cells with the reported changes in permeability of the blood—C.S.F. barrier.
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References
åström, K. E. (1967) On the early development of the isocortex in foetal sheep.Progress in Brain Research 26, 1–59.
Birge, W. J., Rose, A. D., Haywood, J. R. andDoolin, P. F. (1974) Development of the blood-cerebrospinal fluid barrier to proteins and differentiation of cerebrospinal fluid in the chick embryo.Developmental Biology 41, 245–54.
Brightman, M. W. (1968) The intracerebral movement of proteins injected into blood and cerebrospinal fluid of mice.Progress in Brain Research 29, 19–37.
Brightman, M. W. andReese, T. S. (1969) Junctions between intimately apposed cell membranes in the vertebrate brain.Journal of Cell Biology 40, 648–77.
Claude, P. andGoodenough, D. A. (1973) Fracture faces of zonulae occludentes from ‘tight’ and ‘leaky’ epithelia.Journal of Cell Biology 58, 390–400.
Delorme, P. (1972) Différenciation ultrastructurale des jonctiones intercellulaires de l'endothélium des capillaires téléncephaliques chez l'embryon de poulet.Zeitschrift für Zellforschung und mikroskopishe Anatomie 133, 571–82.
Dermietzel, R., Meller, K., Tetzlaff, W. andWaelsch, M. (1977)In vivo andin vitro formation of the tight junctional complex in choroid epithelium.Cell and Tissue Research 181, 427–41.
Doolin, P. F. andBirge, W. J. (1969) Ultrastructural differentiation of the junctional complex of the avian choroidal epithelium.Journal of Comparative Neurology 136, 253–68.
Ducibella, T., Albertini, D. F., Anderson, E. andBiggers, J. D. (1975) The preimplantation mammalian embryo: Characterization of intercellular junctions and their appearance during development.Developmental Biology 45, 231–50.
Dziegielewska, K. M., Evans, C. A. N., Malinowska, D. H., Møllgård, K. Reynolds, J. M., Reynolds, M. L. andSaunders, N. R. (1979) Studies of the development of brain barrier systems to lipid insoluble molecules in fetal sheep.Journal of Physiology (in press).
Elias, P. M. andFriend, D. S. (1976) Vitamin-A-induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentation.Journal of Cell Biology 68, 173–88.
Hull, B. E. andStaehelin, L. A. (1976) Functional significance of the variations in the geometrical organization of tight junction networks.Journal of Cell Biology 68, 688–704.
Larsson, L. (1975) Ultrastructure and permeability of intercellular contacts of developing proximal tubule in the rat kidney.Journal of Ultrastructural Research 52, 100–13.
Martinez-Palomo, A. andErlij, D. (1975) Structure of tight junctions in epithelia with different permeability.Proceedings of the National Academy of Sciences, U.S.A. 72, 4487–91.
Møllgård, K. andSaunders, N. R. (1975) Complex tight junctions of epithelial and of endothelial cells in early foetal brain.Journal of Neurocytology 4, 453–68.
Møllgård, K., Malinowska, D. H., andSaunders, N. R. (1976) Lack of correlation between tight junction morphology and permeability properties in developing choroid plexus.Nature (London) 264, 293.
Møllgård, K., Malinowska, D. H. andSaunders, N. R. (1977) Lack of correlation between tight junction morphology and permeability properties in developing choroid plexus.Nature (London) 267, 183.
Møllgård, K. andSaunders, N. R. (1977) A possible transepithelial pathway via endoplasmic reticulum in fetal sheep choroid plexus.Proceedings of the Royal Society of London B199, 321–6.
Møllgård, K. andRostgaard, J. (1978) Morphological aspects of some sodium transporting epithelia suggesting a transcellular pathway via elements of endoplasmic reticulum.Journal of Membrane Biology 40, 71–89.
Montesano, R., Friend, D. S., Perrelet, A. andOrci, L. (1975)In vivo assembly of tight junctions in fetal rat liver.Journal of Cell Biology 67, 310–9.
Porvaznik, M., Johnson, R. G. andSheridan, J. D. (1976) Intercellular junctions and other cell surface differentiations of H4-IIE hepatoma cells in vitro.Journal of Ultrastructure Research 55, 343–59.
Reese, T. S. andKarnovsky, M. J. (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase.Journal of Cell Biology 34, 204–17.
Schneeberger, E. E., Walters, D. V. andOlver, R. E. (1978) Development of intercellular junctions in the pulmonary epithelium of the fetal lamb.Journal of Cell Science 32, 307–24.
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Møllgård, K., Lauritzen, B. & Saunders, N.R. Double replica technique applied to choroid plexus from early foetal sheep: completeness and complexity of tight junctions. J Neurocytol 8, 139–149 (1979). https://doi.org/10.1007/BF01175557
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DOI: https://doi.org/10.1007/BF01175557