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A special type of small granule-containing cell in the abdominal para aortic region of the frog

  • Published:
Journal of Neurocytology

Summary

Light and electron microscopic studies have been made of a special type of small granule-containing cell (termed Type IV cell) in the frog abdominal para aortic region. These cells contain numerous dense granular vesicles (100–150 nm in diameter) and are considerably smaller (10–20 μ) than neighbouring nerve cells, although they have many features in common with them. They do not resemble chromaffin cells as do Types I, II and III cells. The cell bodies are completely ensheathed by satellite cells and are isolated from neighbouring cells of the same type. Type IV cells have long processes which usually become incorporated in bundles containing 2–20 processes, including some cholinergic nerve fibres, and are loosely enveloped by perineurium. The termination of the processes of Type IV cells do not appear to form efferent synapses on nerve cells at least within the para aortic region or in paravertebral sympathetic ganglia. A close topographical relationship is not found between these processes and blood vessels. It is suggested that the small Type IV granule-containing cells in the frog abdominal para aortic region are not interneurons or neurosecretory cells, but are a special type of sympathetic nerve cell.

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References

  • Chiba, T. andYamauchi, A. (1973) Fluorescence and electron microscopy of the monoamine-containing cells in the turtle heart.Zeitschrift für Zellforschung und mikroskopische Anatomie 140, 25–37.

    Google Scholar 

  • Chiba, T. andWilliams, T. H. (1975) Histofluorescence characteristics and quantification of small intensely fluorescent (SIF) cells in sympathetic ganglia of several species.Cell and Tissue Research 162, 331–41.

    PubMed  Google Scholar 

  • Coupland, R. E. andHopwood, D. (1966) The mechanism of the differential staining reaction for adrenaline- and noradrenaline-storing granules in tissues fixed in glutaraldehyde.Journal of Anatomy 100, 227–43.

    PubMed  Google Scholar 

  • Elfvin, L. G. (1963) The ultrastructure of the superior cervical sympathetic ganglion of the cat. I. The structure of the ganglion cell processes as studied by serial sections.Journal of Ultrastructure Research 8, 403–40.

    Google Scholar 

  • Elfvin, L. G. (1968) A new granule-containing nerve cell in the inferior mesenteric ganglion of the rabbit.Journal of Ultrastructure Research 22, 37–44.

    PubMed  Google Scholar 

  • Elfvin, L. G. (1971a) Ultrastructural studies on the synaptology of the inferior mesenteric ganglion of the cat. I. Observations on the cell surface of the postganglionic perikarya.Journal of Ultrastructure Research 37, 411–25.

    PubMed  Google Scholar 

  • Elfvin, L. G. (1971b) Ultrastructural studies, on the synaptology of the inferior mesenteric ganglion of the cat. III. The structure and distribution of the axodendritic and dendrodendritic contacts.Journal of Ultrastructure Research 37, 432–48.

    PubMed  Google Scholar 

  • Elfvin, L. G., Hökfelt, T. andGoldstein, M. (1975) Fluorescence microscopical, immunohistochemical and ultrastructural studies on sympathetic ganglia of the guinea-pig, with special reference to the SIF cells and their catecholamine content.Journal of Ultrastructure Research 51, 377–96.

    PubMed  Google Scholar 

  • Fujimoto, S. (1967) Some observations on the fine structure of the sympathetic ganglion of the toad,Bufo vulgaris japonicus. Archivum histologicum japonicum 28, 313–35.

    Google Scholar 

  • Grillo, M. A. (1966) Electron microscopy of sympathetic tissues.Pharmacological Reviews 18, 387–99.

    PubMed  Google Scholar 

  • Grillo, M. A., Jacobs, L. andComroe, J. H. Jr. (1974) A combined fluorescence histochemical and electron microscopic method for studying special monoamine-containing cells (SIF cells).Journal of Comparative Neurology 153, 1–14.

    PubMed  Google Scholar 

  • Hill, C. E., Watanabe, H. andBurnstock, G. (1975) Distribution and morphology of amphibian extra-adrenal chromaffin tissue.Cell and Tissue Research 160, 371–87.

    PubMed  Google Scholar 

  • Hopsu, V. K. andMäkinen, E. O. (1966) Two methods for the demonstration of noradrenaline-containing adrenal medullary cells.Journal of Histochemistry and Cytochemistry 14, 434–5.

    PubMed  Google Scholar 

  • Libet, B. (1970) Generation of slow inhibitory and excitatory postsynaptic potentials.Federation Proceedings 29, 1945–56.

    PubMed  Google Scholar 

  • Libet, B. andTosaka, T. (1970) Dopamine as a synaptic transmitter and modulator in sympathetic ganglia: A different mode of synaptic action.Proceedings of the National Academy of Science, U.S.A. 67, 667–73.

    Google Scholar 

  • Libet, B. andOwman, C. (1974) Concomitant changes in formaldehyde-induced fluorescence of dopamine interneurones and in slow inhibitory post-synaptic potentials of the rabbit superior cervical ganglion, induced by stimulation of the preganglionic nerve or by a muscarinic agent.Journal of Physiology 237, 635–62.

    PubMed  Google Scholar 

  • Matthews, M. R. andRaisman, G. (1969) The ultrastructure and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion.Journal of Anatomy 105, 255–82.

    PubMed  Google Scholar 

  • Pick, J. (1963) The submicroscopic organisation of the sympathetic ganglion in the frog (Rana pipiens).Journal of Comparative Neurology 120, 409–62.

    PubMed  Google Scholar 

  • Pick, J. (1970)The autonomic nervous system. Morphological comparative, clinical and surgical aspects. Philadelphia-Toronto: J. B. Lippincott Company.

    Google Scholar 

  • Piezzi, R. S. andRodriguez Echandia, E. L. (1968) Studies on the pararenal ganglion of the toadBufo arenarum Hensel. I. Its normal fine structure and histochemical characteristics.Zeitschrift für Zellforschung und mikroskopische Anatomie 88, 180–6.

    Google Scholar 

  • Santer, R. M., Lu, K. S., Lever, J. D. andPresley, R. (1975) A study of the distribution of chromaffin-positive (CH+) and small intensely fluorescent (SIF) cells in sympathetic ganglia of the rat at various ages.Journal of Anatomy 119, 589–99.

    PubMed  Google Scholar 

  • Siegrist, G., Dolivo, M., Dunant, Y., Foroglou-Kerameus, C., Deribaupierre, F. andRouiller, C. (1968) Ultrastructure and function of the chromaffin cells in the superior cervical ganglion of the rat.Journal of Ultrastructure Research 25, 381–407.

    PubMed  Google Scholar 

  • Taxi, J. (1961) Etude de l'ultrastructures des zones synaptiques dans les ganglions sympathetiques de la grenouille.Compte rendu de l'Académie des sciences, Paris 252, 174–6.

    Google Scholar 

  • Taxi, J. (1965) Contribution á l'étude des connexions des neurones moteurs de système nerveux autonome.Annales des Sciences Naturelles, Zoologie et Biologie Animale, Paris, Ser.12.7, 413–674.

    Google Scholar 

  • Uchizono, K. andOhsawa, K. (1973) Morpho-physiological consideration on synaptic transmission in the amphibian sympathetic ganglion.Acta Physiologica Polonica 24, 205–14.

    PubMed  Google Scholar 

  • Watanabe, H. (1971) Adrenergic nerve elements in the hypogastric ganglion of the guinea pig.American Journal of Anatomy 130, 305–30.

    PubMed  Google Scholar 

  • Watanabe, H. andBurnstock, G. (1976) Junctional subsurface organs in frog sympathetic ganglion cells.Journal of Neurocytology 5, 125–36.

    PubMed  Google Scholar 

  • Weight, F. F. andPadjen, A. (1973) Acetylcholine and slow synaptic inhibition in frog sympathetic ganglion cell.Brain Research 55, 225–8.

    PubMed  Google Scholar 

  • Weitsen, H. A. andWeight, F. F. (1973) Chromaffin cells in the frog sympathetic ganglion: morphology not consistent with role in generation of synaptic potentials.Anatomical Record 175, 467.

    Google Scholar 

  • Williams, T. H. (1967a) Electron microscopic evidence for an autonomic interneuron.Nature 214, 309–10.

    Google Scholar 

  • Williams, T. H. (1967b) The question of the intraganglionic (connector) neuron of the autonomic nervous system.Journal of Anatomy 101, 603–4.

    Google Scholar 

  • Williams, T. H. andPalay, S. L. (1969) Ultrastructure of the small neurons in the superior cervical ganglion.Brain Research 15, 17–34.

    PubMed  Google Scholar 

  • Williams, T. H., Black., A. C. Jr., Chiba, T. andBhalla, R. A. (1975) Morphology and biochemistry of small, intensely fluorescent cells of sympathetic ganglia.Nature 256, 315–7.

    PubMed  Google Scholar 

  • Yamamoto, T. (1963) Some observations on the fine structure of the sympathetic ganglion of bullfrog.Journal of Cell Biology 16, 159–70.

    PubMed  Google Scholar 

  • Yamauchi, A., Fujimaki, Y. andYokota, R. (1975) Reciprocal synapses between cholinergic postganglionic axon and adrenergic interneuron in the cardiac ganglion of the turtle.Journal of Ultrastructure Research 50, 49–57.

    Google Scholar 

  • Yokota, R. (1973) The granule-containing cell somata in the superior cervical ganglion of the rat, as studied by a serial sampling method for electron microscopy.Zeitschrift für Zellforschung und mikroskopische Anatomie 141, 331–45.

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan

    Hiroshi Watanabe

  2. Department of Anatomy and Embryology, University College London, Gower Street, London, England

    Geoffrey Burnstock

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  1. Hiroshi Watanabe
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  2. Geoffrey Burnstock
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Watanabe, H., Burnstock, G. A special type of small granule-containing cell in the abdominal para aortic region of the frog. J Neurocytol 5, 465–478 (1976). https://doi.org/10.1007/BF01181651

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  • DOI: https://doi.org/10.1007/BF01181651

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