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Semiquantitative determination of micro amounts of iron, copper, cobalt, nickel, and zinc in blood and tissue by circular thin-layer chromatography

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Summary

A semiquantitative method for the determination of iron, copper, cobalt, nickel and zinc is described which is based on circular thin-layer chromatographic technique. The error is minimized to ± 5% and interference of about 40 metal ions is removed by solvent extraction. The application of the method has been demonstrated by the determination of these metal ions in human blood and tissue and the method is suggested for the study of airborne particulates.

Zusammenfassung

Ein halbquantitatives Verfahren zur Bestimmung von Eisen, Kupfer, Kobalt, Nickel und Zink auf dünnschicht-ringchromatographischem Wege wurde beschrieben. Der Fehler wurde auf ±5% eingeschränkt und die störende Wirkung von etwa 40 Metallionen durch Lösungsmittelextraktion beseitigt. Die Anwendbarkeit des Verfahrens für die Bestimmung in menschlichem Blut und Gewebe wurde gezeigt bzw. für die Untersuchung atmosphärischer Verunreinigungen empfohlen.

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Authors and Affiliations

  1. West Regional Laboratories, Pakistan Council of Scientific and Industrial Research, Lahore, West Pakistan

    M. H. Hashmi, Farhat Rafique Chughtai, N. A. Chughtai, Abdur Rashid & Maqbool Ahmad Shahid

Authors
  1. M. H. Hashmi
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  2. Farhat Rafique Chughtai
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  3. N. A. Chughtai
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  4. Abdur Rashid
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  5. Maqbool Ahmad Shahid
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Hashmi, M.H., Chughtai, F.R., Chughtai, N.A. et al. Semiquantitative determination of micro amounts of iron, copper, cobalt, nickel, and zinc in blood and tissue by circular thin-layer chromatography. Mikrochim Acta 56, 712–718 (1968). https://doi.org/10.1007/BF01216909

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  • DOI: https://doi.org/10.1007/BF01216909

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