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Effects of centrally administered neuropeptide Y (NPY) and NPY13–36 on the brain monoaminergic systems of the rat

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Summary

The effects of centrally administered NPY on the brain monoamine systems were investigated in the rat. Neuropeptide Y (0.2–5.0 nmol), its C-terminal 13–36 amino acid (a.a.) fragment, NPY13–36 (0.4–10.0 nmol), or saline were injected into the right lateral cerebral ventricle of unrestrained rats. After l h the animals were decapitated, and the brains were taken out. Two cortical regions (‘frontal’ and ‘parietal’), the striatum, the hypothalamus, and the brain stem were dissected out. The tissue contents of noradrenaline (NA), dopamine (DA) and serotonin (5-HT), as well as of their major metabolites, 3-methoxy-4-hydroxy-phenylethylené glycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxy-indole acetic acid (5-HIAA) were measured. The most consistent finding was a dose-related increase of both DA and DOPAC levels after treatment with NPY. This effect was reproduced by NPY13–36 in cortical tissue, whereas, in the sub-cortical regions, NPY13–36 only reproduced the effects of NPY on the DOPAC levels. Less consistent effects were found on the NA systems, in which NA levels showed a tendency to increase following low, and decrease after high doses of NPY. These effects were largely reproduced by NPY13–36. In addition, NPY increased tissue levels of MHPG in frontal cortical tissue in a dose-related manner. The brain 5-HT systems were not affected.

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References

  • Agnati LF, Fuxe K, Benfenati F, Battistini N, Härfstrand A, Tatemoto K, Hökfelt T, Mutt V (1983) Neuropeptide Y in vitro selectivity increases the number of alpha-2-adrenergic binding sites in membranes of the medulla oblongata of the rat. Acta Physiol Scand 118: 293–295

    Google Scholar 

  • Albers HE, Ferris CF, Leeman SE, Goldman BD (1984) Avian pancreatic polypeptide phase shifts hamster circadian rhythms when microinjected into the suprachiasmatic region. Science 223: 833–835

    Google Scholar 

  • Beal MF, Frank RC, Ellison DW, Martin JB (1986) The effect of neuropeptide Y on striatal catecholamines. Neurosci Lett 71: 118–123

    Google Scholar 

  • Carlsson A (1978) Mechanism of action of neuroleptic drugs. In: Lipton MA, DiMascio A, Killam KF (eds) Psychopharmacology: a generation of progress. Raven Press, New York, pp 1057–1070

    Google Scholar 

  • Chronwall BM, Chase TN, O'Donohue TL (1984) Coexistence of neuropeptide Y and somatostatin in rat and human cortical and rat hypothalamic neurons. Neurosci Lett 52: 213–217

    Google Scholar 

  • Chronwall BM, DiMaggio DA, Massari VJ, Pickel VM, Ruggiero DA, O'Donohue TL (1985) The anatomy of neuropeptide-Y-containing neurons in rat brain. Neuroscience 15: 1159–1181

    Google Scholar 

  • Colmers WF, Lukowiak K, Pittman QJ (1985) Neuropeptide Y reduces orthodromically evoked population spike in rat hippocampal CA 1 by a possibly presynaptic mechanism. Brain Res 346: 404–408

    Google Scholar 

  • Eriksson BM, Persson BA (1982) Determination of catecholamines in rat heart tissue and plasma samples by liquid chromatography with electrochemical detection. J Chromatogr 228: 143–154

    Google Scholar 

  • Everitt BJ, Hökfelt T, Terenius L, Tatemoto K, Mutt V, Goldstein M (1984) Differential co-existence of neuropeptide Y (NPY)-like immunoreactiviy with catecholamines in the central nervous system of the rat. Neuroscience 11: 443–462

    Google Scholar 

  • Fuxe K, Agnati LF, Härfstrand A, Zini I, Tatemoto K, Pich EM, Hökfelt T, Mutt V, Terenius L (1983) Central administration of neuropeptide Y induces hypotension, bradypnea and EEG synchronization in the rat. Acta Physiol Scand 118: 189–192

    Google Scholar 

  • Haas HL, Hermann A, Greene RW, Chan-Palay V (1987) Action and location of neuropeptide tyrosine (Y) on hippocampal neurons of the rat in slice preparations. J Comp Neurol 257: 208–215

    Google Scholar 

  • Heilig M, Murison R (1987) Intracerebroventricular neuropeptide Y suppresses open field and home cage activity in the rat. Regul Pept 19: 221–231

    Google Scholar 

  • Heilig M, Murison R (1987) Intracerebroventricular neuropeptide Y protects against stressinduced gastric erosion in the rat. Eur J Pharmacol 137: 127–129

    Google Scholar 

  • Heilig M, Wahlestedt C, Ekman R, Widerlöv E (1988) Antidepressant drugs increase the concentration of neuropeptide Y (NPY)-like immunoreactivity in the rat brain. Eur J Pharmacol 147: 465–467

    Google Scholar 

  • Heilig M, Wahlestedt C, Widerlöv E (1988) Neuropeptide Y (NPY) induced activity suppression in the rat: evidence for NPY receptor heterogeneity and for interaction with alpha-adrenergic receptors. Eur J Pharmacol 157: 205–213

    Google Scholar 

  • Heilig M, Söderpalm B, Engel JA, Widerlöv E (1989) Centrally administered neuropeptide Y (NPY) produces anxiolytic-like effects in animal anxiety models. Psychopharmacology (in press)

  • Hendry SH, Jones EG, DeFelipe J, Schmechel D, Brandon C, Emson PC (1984) Neuropeptide-containing neurons of the cerebral cortex are also GABAergic. Proc Natl Acad Sci U.S.A. 81: 6526–6530

    Google Scholar 

  • Härfstrand A, Eneroth P, Agnati L, Fuxe K (1987) Further studies on the effects of central administration of neuropeptide Y on neuroendocrine function in the male rat: relationship to hypothalamic catecholamines. Regul Pept 17: 167–179

    Google Scholar 

  • Iversen SD (1977) Brain dopamine systems and behaviour. In: Iversen LL, Iversen SD, Snyder SH (eds) Handbook of Psychopharmacology, vol 8. Plenum Press, New York, pp 333–384

    Google Scholar 

  • Kalra SP, Crowley WR (1984) Norepinephrine-like effects of neuropeptide Y on LH release in the rat. Life Sci 35: 1173–1176

    Google Scholar 

  • Kerkerian L, Bosler O, Pelletier G, Nieoullon A (1986) Striatal neuropeptide Y neurones are under the influence of the nigrostriatal dopaminergic pathway: immunohistochemical evidence. Neurosci Lett 66: 106–122

    Google Scholar 

  • Levine AS, Morley JE (1984) Neuropeptide Y: a potent inducer of consummatory behavior in rats. Peptides 5: 1025–1029

    Google Scholar 

  • Lundberg JM, Terenius L, Hökfelt T, Martling CR, Tatemoto K, Mutt V, Polak J, Bloom S, Goldstein M (1982) Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand 116: 477–480

    Google Scholar 

  • Modigh K (1987) Antidepressant drugs in anxiety disorders. Acta Psychiatr Scand 76 [Suppl 335]: 51–57

    Google Scholar 

  • Monti JM (1982) Catecholamines and the sleep-wake cycle. I. EEG and behavioural arousal. Life Sci 30: 1145–1157

    Google Scholar 

  • Redmond DE, Huang YH (1979) New evidence for a locus coeruleus-norepinephrine connection with anxiety. Life Sci 25: 2149–2162

    Google Scholar 

  • Siever LJ, Davies KL (1985) Overview: towards a dysregulation hypothesis of depression. Am J Psychiatry 142: 1017–1031

    Google Scholar 

  • Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y—a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature 296: 659–666

    Google Scholar 

  • Undén A, Tatemoto K, Mutt V, Bartfai T (1984) Neuropeptide Y receptor in the rat brain. Eur J Biochem 145: 525–530

    Google Scholar 

  • Vallejo M, Carter DA, Biswas S, Lightman SL (1987) Neuropeptide Y alters monoamine turnover in the rat brain. Neurosci Lett 73: 155–160

    Google Scholar 

  • Wahlestedt C, Yanaihara N, Håkanson R (1986) Evidence for different pre- and post-junctional receptors for neuropeptide Y and related peptides. Regul Pept 13: 307–318

    Google Scholar 

  • Wahlestedt C, Skagerberg G, Ekman R, Heilig M, Sundler F, Håkanson R (1987) Neuropeptide Y (NPY) in the area of the hypothalamic paraventricular nucleus activates the pituitary-adrenocortical axis in the rat. Brain Res 417: 33–38

    Google Scholar 

  • Wahlestedt C, Edvinson L, Ekblad E, Håkanson R (1988) Effects of neuropeptide Y at sympathetic neuroeffector junctions: existence of Y1 and Y2 receptors. In: Nobin A, Owman C (eds) Neuronal Messengers in Vascular Function. Elsevier, Amsterdam

    Google Scholar 

  • Wahlestedt C, Ekman R, Widerlöv E (1989) Neuropeptide Y (NPY) and the central nervous system: distribution, effects and possible relationship to neurological and psychiatric disorders. Prog Neuropsychopharmacol Biol Psychiatry 13: 31–54

    Google Scholar 

  • Westlind-Danielsson A, Undén A, Abens J, Andell S, Bartfai T (1987) Neuropeptide Y receptors and the inhibition of adenylate cyclase in the human frontal and temporal cortex. Neurosci Lett 74: 237–242

    Google Scholar 

  • Widerlöv E, Lewander T (1978) Inhibition of the in vivo biosynthesis and changes of catecholamine levels in rat brain after alpha-Methyl-p-Tyrosine; time- and dose-response relationships. Naunyn-Schmiedebergs Arch Pharmacol 304: 111–123

    Google Scholar 

  • Widerlöv E, Lindström LH, Wahlestedt C, Ekman R (1988) Neuropeptide Y and peptide YY as possible cerebrospinal markers for major depression and schizophrenia, respectively. J Psychiatr Res 22 (1): 69–79

    Google Scholar 

  • Widerlöv E, Heilig M, Ekman R, Wahlestedt C (1989) Neuropeptide Y—possible involvement in depression and anxiety. In: Mutt V, Fuxe K, Hökfelt T (eds) Nobel Conference on NPY. Raven Press, New York

    Google Scholar 

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Author notes

  1. E. Widerlöv

    Present address: Department of Psychiatry and Neurochemistry, University of Lund, Lund, Sweden

Authors and Affiliations

  1. Department of Psychiatry and Neurochemistry, University of Lund, Lund, Sweden

    M. Heilig, L. Vècséi, C. Wahlestedt & Ch. Ailing

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  1. M. Heilig
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  2. L. Vècséi
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  3. C. Wahlestedt
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  4. Ch. Ailing
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  5. E. Widerlöv
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Heilig, M., Vècséi, L., Wahlestedt, C. et al. Effects of centrally administered neuropeptide Y (NPY) and NPY13–36 on the brain monoaminergic systems of the rat. J. Neural Transmission 79, 193–208 (1990). https://doi.org/10.1007/BF01245130

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