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In vivo trapping of hydroxyl free radicals in the striatum utilizing intracranial microdialysis perfusion of salicylate: effects of MPTP, MPDP+, and MPP+

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Summary

Increased formation of hydroxyl free radicals (·OH) reflected by ·OH adduct of salicylate in brain dialysate was demonstrated during the sustained (more than 2 hours) dopamine overflow elicited by 75 nmol of 1-methyl-4-phenyldihydropyridine (MPDP+) and 1-methyl-4-phenylpyridinium (MPP+) in the rat striatum. Owing to its weak dopamine releasing action, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) did not significantly increase the ·OH formation. This data suggests that sustained elevation of dopamine in the extracellular fluid elicited by MPTP analogues can be auto-oxidized, which in turn leads (possibly by indirect mechanisms) to the formation of cytotoxic ·OH free radicals near the nigrostriatal terminals.

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  1. C. C. Chiueh

    Present address: National Institute of Mental Health, Bethesda, Md, USA

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  1. National Institute of Neurological Disorders and Stroke, Bethesda, Md, USA

    T. Obata

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  1. T. Obata
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  2. C. C. Chiueh
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Obata, T., Chiueh, C.C. In vivo trapping of hydroxyl free radicals in the striatum utilizing intracranial microdialysis perfusion of salicylate: effects of MPTP, MPDP+, and MPP+ . J. Neural Transmission 89, 139–145 (1992). https://doi.org/10.1007/BF01245361

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  • DOI: https://doi.org/10.1007/BF01245361

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