Summary
Aqueous extracts of sheep pineal bodies and sheep cerebral cortex were separated on Sephadex G-25 and G-10. Several distinct peaks could be distinguished showing excitation and fluorescence maxima resembling those of indoles. However, in sheep pineal extract one peak was observed with different excitation and fluorescence maxima which could not be observed in sheep cerebral cortex. Using two-dimensional thinlayer chromatography a substance designated A was isolated and purified, showing excitation maxima at 298 nm and 358 nm, and a fluorescence maximum at 440 nm. Rf values of this substance A in 7 different solvent systems in thinlayer chromatography are presented. The results of paper electrophoresis experiments are also described.
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Zurburg, W., Ebels, I. Separation of pineal extracts by gelfiltration. J. Neural Transmission 35, 117–124 (1974). https://doi.org/10.1007/BF01250739
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DOI: https://doi.org/10.1007/BF01250739