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Autoradiographic localization of dopamine D1 and D2 receptors in the brain of several mammalian species

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Summary

Dopamine D 1 and D 2 receptor distributions were studied in the brain of the mouse, rat, guinea pig, cat and monkey by means of in vitro quantitative autoradiography using [3H]SCH 23390 and [3H]CV 205–502 to label D 1 and D 2 subtypes respectively.

The distribution of both subtypes of receptors was similar within the basal ganglia of all species investigated. The highest densities for both subtypes were found in the nucleus caudatus, putamen, nucleus accumbens, olfactory tubercle and substantia nigra.

Outside of the basal ganglia, differences in the distribution of both receptors were found among the species examined in regions such as cerebellum, cortex, hippocampus, superior colliculus and olfactory bulb.

In all species D 1 receptor densities were higher than those of D 2. The absolute amount of both subtypes, however, varied among species.

These results indicate that dopamine receptor distribution is well preserved in the basal ganglia during evolution, although differences among species exist in their distribution outside the basal ganglia and their absolute amount.

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Abbreviations

A :

corpus amygdalae

Acc :

nucleus accumbens

CA 1 :

CA 1 subfield of the hippocampus

CA 2 :

CA 2 subfield of the hippocampus

CgC :

cingular cortex

Cls :

claustrum

Cd :

nucleus caudatus

CPu :

caudate-putamen

DA :

dopamine

DG :

dentate gyrus of the hippocampus

EP :

nucleus entopeduncularis

GP :

globus pallidus

GPl :

globus pallidus, pars lateralis

GPm :

globus pallidus, pars medialis

gr :

granular layer of the cerebellum

IP :

nucleus interpeduncularis

LS :

lateral septum

mol :

molecular layer of the cerebellum

OT :

olfactory tubercle

Pk :

Purkinje cell layer of the cerebellum

Pu :

putamen

SC :

superior colliculus

snc :

substantia nigra, pars compacta

snr :

substantia nigra, pars reticulata

VTA :

area ventralis tegmentalis

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  1. J. M. Palacios

    Present address: Preclinical Research, Sandoz AG, CH-4002, Basel, Switzerland

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  1. Preclinical Research, Sandoz AG, CH-4002, Basel, Switzerland

    M. Camps & P. H. Kelly

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Camps, M., Kelly, P.H. & Palacios, J.M. Autoradiographic localization of dopamine D1 and D2 receptors in the brain of several mammalian species. J. Neural Transmission 80, 105–127 (1990). https://doi.org/10.1007/BF01257077

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