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Oncorna-viral information in human glioblastoma

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Summary

Two of the main biochemical features characteristic for oncogenic RNA (oncorna) viruses have been detected in human glioblastoma. In all tumors tested so far a RNA instructed DNA polymerase (“reverse transcriptase”) activity was present which exhibited the criteria specific for oncorna viruses. It was stimulable by the synthetic polynucleotide poly-rA: oligo-dT, it was almost insensitive to actinomycin D but very sensitive to ethidiumbromide. Using the simultaneous detection test, a high molecular weight RNA species was found in the subcellular fraction with the highest reverse transcriptase activity.

As revealed by electron microscopy, this subcellular fraction contains particles of a morphology similar to oncogenic RNA viruses of the C-type.

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  1. G. D. Birkmayer

    Present address: Departments of Cell Biology and Neurosurgery, University of Munich, Federal Republic of Germany

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  1. Departments of Cell Biology and Neurosurgery, University of Munich, Federal Republic of Germany

    F. Miller & F. Marguth

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  1. G. D. Birkmayer
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  2. F. Miller
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  3. F. Marguth
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Birkmayer, G.D., Miller, F. & Marguth, F. Oncorna-viral information in human glioblastoma. J. Neural Transmission 35, 241–254 (1974). https://doi.org/10.1007/BF01258955

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  • DOI: https://doi.org/10.1007/BF01258955

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