Summary
A screening method for detecting volatile hydrocarbons in blood has been developed using gas chromatography/mass spectrometry with a wide-bore capillary column and a headspace method. Toluene-d8 and indan were used as the internal standards for quantitative analysis. Hydrocarbons with retention indices from 600 to 1200 were simultaneously and quantitatively detected in relatively low concentrations (0.01 μg/ml) in reconstructed ion chromatography. This method could prove useful in forensic cases in which urgent examination of complex hydrocarbon mixtures, e.g. petroleum components, is required.
Zusammenfassung
Es wird über eine Screeningmethode zum Nachweis flüchtiger Kohlenwasserstoffe im Blut mittels Gaschromatographie/Massen-spektrometrie berichtet, wobei wide-bore Kapillaren und die Headspace-technik eingesetzt wurden. Toluol-d8 und Indan dienten als interne Standards bei der quantitativen Analyse. Kohlenwasserstoffe mit Retentionsindi-zes zwischen 600 und 1200 konnten mit der Ionenchromatographie simultan und quantitativ noch in relativ geringen Konzentrationsbereichen bis 0.01 μg/ ml bestimmt werden. In der Praxis ist die Methode nützlich, wenn es in forensischen Fällen darum geht, komplexe Kohlenwasserstoffgemische (wie z.B. Petroleum) rasch zu analysieren.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nagata T, Kageura M, Hara K (1983) Signification of inflammable substances in burned bodies. In: Proceedings of the Second Scandinavian Conference on Forensic Science, pp 77–80
Nagata T, Kageura M, Hara K, Totoki K (1977) Gas chromatographic-mass spectrometric determination of gasoline in blood (in Japanese with English summary). Jpn J Leg Med 31:136–139
Kimura K, Nagata T, Hara K, Kageura M (1988) Gasoline and kerosene components in blood — A forensic analysis. Hum Toxicol 7:299–305
Kobus HJ, Kirkbride KP, Maehly A (1987) An adsorption sampling method combined with capillary column gas chromatography and cryogenic focussing for trace analysis of vol-atile organic compounds. J Forensic Sci Soc 27:307–314
Davoli E, Cappellini L, Airoldi L, Fanelli R (1986) Trace analysis of methanol in rat serum by headspace high resolution gas chromatography/selected ion monitoring. J Chromatogr Sci 24:113–116
Pfaffli P, Svartling N (1985) Assay of methyl methacrylate in blood samples by headspace capillary gas chromatography. J Chromatogr 345:386–389
Termonia M, Meyer AD, Wybauw M, Jacobs H (1982) Analysis of C1–C3 alcohols in blood by high resolution headspace gas chromatography. HRC & CC 5:377–378
Michael LC, Erickson MD, Parks SP, Pellizzari ED (1980) Volatile environmental pollut-ants in biological matrices with a headspace purge technique. Anal Chem 52:1836–1841
Zlatkis A, Lichtenstein HA, Tishbee A, Bertsch W, Shunbo F, Leibich HM (1973) Concentration and analysis of volatile urinary metabolites. J Chromatogr Sci 11:299–302
Penton Z (1987) Gas-chromatographic determination of ethanol in blood with 0.53-mm fused-silica open tubular columns. Clin Chem 33:2094–2095
Newton B, Foery RF (1984) Retention indices and dual capillary gas chromatography for rapid identification of sedative hypnotic drugs in emergency toxicology. J Anal Toxicol 8:129–134
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morinaga, M., Hara, K., Kageura, M. et al. A simple, rapid and simultaneous analysis of complex volatile hydrocarbon mixtures in blood using gas chromatography/mass spectrometry with a wide-bore capillary column. Z Rechtsmed 103, 567–572 (1990). https://doi.org/10.1007/BF01261420
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01261420