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Electro-enhancement of division of plant protoplast-derived cells

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Summary

Electric field pulses, ranging from 250 to 2000 V and of 10 to 50 μsec duration, were assessed for their effect on the growth in culture of isolated protoplasts ofGlycine canescens, Prunus avium × pseudocerasus, Pyrus communis, Solanum dulcamara andSolanum viarum. Three successive voltage pulses between 250 and 1000 V caused a small decrease in protoplast viability, but promoted cell division and enhanced significantly the plating efficiency. A higher percentage of electro-pulsed protoplasts showed sustained growth in culture to the microcallus stage compared to untreated protoplasts. The rate of cell division was also stimulated in electro-treated protoplasts. These observations are discussed in relation to present knowledge of the effects of electrical treatments on plant and animal cells.

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK

    E. L. Rech, S. J. Ochatt, P. K. Chand (Post Graduate), J. B. Power & M. R. Davey

  2. Centro Nacional de Recursos Geneticos e Biotecnologia (EMBRAPA), Brasilia, Brazil

    E. L. Rech

  3. Centro de Ecofisiologia Vegetal (CONICET), Buenos Aires, Argentina

    S. J. Ochatt

  4. Department of Botany, Utkal University, Bhubaneshwar, India

    P. K. Chand (Post Graduate)

Authors
  1. E. L. Rech
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  2. S. J. Ochatt
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  3. P. K. Chand
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  4. J. B. Power
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  5. M. R. Davey
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Rech, E.L., Ochatt, S.J., Chand, P.K. et al. Electro-enhancement of division of plant protoplast-derived cells. Protoplasma 141, 169–176 (1987). https://doi.org/10.1007/BF01272899

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  • DOI: https://doi.org/10.1007/BF01272899

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