Summary
At telophase in the diatomPinnularia, the two half spindles that comprise the central spindle, separate and then disassemble unidirectionally from the end formerly in the central overlap, back to the pole (Soranno andPickett-Heaps 1982). The metabolic inhibitors dinitrophenol plus deoxyglucose were applied to cells at telophase, depleting their ATP levels at the early stages of half-spindle disassembly; the cells were maintained in this state for 5 minutes, before the inhibitors were washed out. Disassembly of the half spindles, as judged from their birefringence, ceased in ATP-depleted conditions, and recommenced soon after the inhibitors were removed, going to completion quite rapidly.
We conclude that disassembly of these MTsin vivo requires energy, probably in the form of ATP.
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Hinz, I., Spurck, T.P. & Pickett-Heaps, J.D. Metabolic inhibitors and mitosis: III. Effects of dinitrophenol on spindle disassembly inPinnularia . Protoplasma 132, 85–89 (1986). https://doi.org/10.1007/BF01275794
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DOI: https://doi.org/10.1007/BF01275794