Summary
The effect of some chemicals (cycloheximide, puromycin, actinomycin D, ribonuclease, cytochalasin B, caffeine, colchicine IPC, CIPC and glusulase) upon morphogenesis inMicrasterias was studied, and some of our results are compared to studies by other investigators. Cycloheximide and actinomycin D inhibited differentiation of the expanding primary wall and the cells exhibited signs of recovery if the exposure to the drug was short. Puromycin had little effect upon morphogenesis. Cytochalasin B and caffeine drastically disrupted differentiation and expansion of the primary wall apparently by inhibiting wall secretion. The effect of cycloheximide and glusulase upon wall secretion itself has been investigated. The patterning of accumulated wall material, characteristic of deposition under reduced turgor, was at least partially disrupted during exposure to cycloheximide. Cells were also centrifuged so as to force the chloroplast against the plasmalemma, thereby presumably interfering with wall secretion and stopping cell expansion and differentiation.
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Tippit, D.H., Pickett-Heaps, J.D. Experimental investigations into morphogenesis inMicrasterias . Protoplasma 81, 271–296 (1974). https://doi.org/10.1007/BF01275816
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DOI: https://doi.org/10.1007/BF01275816