Summary
Heterospecific chimeralSolanum nigrum (+)Solanum tuberosum plants were obtained by cell grafting in protoplast co-cultures. Periclinal, sectorial, and mericlinal chimeras have been identified by various morphological and cytological characteristics.
Morphogenesis predominantly began in periclinal chimeral organization. Cells of different species have been found to be interconnected by secondary plasmodesmata. Plantlets of all chimeral lines were grown to flowering under tissue culture conditions and some also in the greenhouse. Aspects of organogenesis and interspecific cooperation are discussed.
Similar content being viewed by others
Abbreviations
- B 5:
-
culture medium (Gamborg et al. 1968) supplemented with 2.5μM 6-benzyladenin
- L1, L2, L3:
-
epidermis (L1), subepidermal layer (L2), core (L3)
- MS:
-
culture medium (Murashige andSkoog 1962)
- n, n w :
-
symbols used for the indication ofSn-F (n) orSn-F-W2 (n w ) tissue in L1, L2 or L3
- Sn :
-
Solanum nigrum
- Sn-F:
-
is an atrazine-resistant biotype
- Sn-F-W2:
-
is a plastid mutant malbino derivative ofSn-F
- St :
-
Solanum tuberosum
- St-H2258:
-
is a dihaploid clone
- t :
-
symbol used the indication ofSt-H2258 tissue in L1, L2 or L3
- V-KM:
-
culture medium (Binding and Nehls 1977)
References
Baur E (1909 a) Das Wesen und die Erblichkeitsverhältnisse der „Varietas albomarginatae hort“ vonPelargonium zonale. Z Vererbungsl 1: 330–351
— (1909 b) Pfropfbastarde, Periklinalchimären und Hyperchimären. Ber Dtsch Bot Ges 27: 603–605
— (1910) Pfropfbastarde. Biol Zbl 30: 497–514
— (1911) Entgegnungen. Z Bot 3: 198–202
Binding H (1984) Aufzucht von Pflanzen aus isolierten Protoplasten und Fusionskörpern. In: Mitteilungsband —Kurzfassungen der Beiträge, Botaniker Tagung in Wien 1984, p 61
-Kollmann R (1985) Regeneration of protoplasts. In:Schäfermenuhr (ed)In Vitro Techniques —Propagation and Long Term Storage, Comm Europ Commun, Nijhoff, Junk Publ, pp 93–99
—,Mordhorst G (1984) HaploidSolanum dulcamara L.: Shoot culture and plant regeneration from isolated protoplast. Plant Sci Lett 35: 77–79
—,Nehls R (1977) Regeneration of isolated protoplasts to plants inSolanum dulcamara L. Z Pflanzenphysiol 85: 279–280
— —,Schieder O, Sopory SK, Wenzel G (1978) Regeneration of mesophyll protoplasts isolated from dihaploid clones ofSolanum tuberosum L. Physiol Plant 43: 52–54
—,Jain SM, Finger J, Mordhorst G, Nehls R, Gressel J (1982) Somatic hybridization of an atrazine resistant biotype ofSolanum nigrum withSolanum tuberosum. 1. Clonal variation in morphology and in atrazine sensitivity. Theor Appl Genetics 63: 273–277
—,Görschen E, Jörgensen J, Krumbiegel-Schroeren G, Ling HQ, Mordhorst G, Witt D, Kollmann R (1986) Protoplast regeneration and fusion in agarose lenses. In:Smers DA, GenGenbach BG, Biesboer DD, Hackett WP, Green CE (eds) Abstr VI Internatl Congr Plant Tissue and Cell Culture, Univ Minnesota, Minneapolis, p 318
Buder J (1911) Studien anLaburnum adami. II. Allgemeine anatomische Analyse des Mischlings und seiner Stammpflanzen. Z Vererbungsl 5: 209–284
Burgess J (1972) The occurrence of plasmodesmata-like structures in a non-division wall. Protoplasma 74: 449–458
Carlson PS, Chaleff RS (1975) Heterogeneous associations of cells formed in vitro. InLedoux L (ed) Genetic mainpulations with plant material. Plenum Press, New York, p 245–261
Fabergé AC, Oliver RM (1974) Methylamine tungstate, a new negative stain. I. Microscopie 20: 241–246
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Gasquez J, Barralis G (1979) Mise en évidence de la résistance aux triazines chezSolanum nigrum L. etPolygonum lapathifolium L. par observation de la fluorescence des feuilles isolées. CR Acad Sci (Paris) Sec D 288: 1391–1393
Kollmann R, Glockmann C (1985) Studies on graft unions I. Plasmodesmata between cells of plants belonging to different unrelated taxa. Protoplasma 124: 224–235
—,Yang S, Glockmann C (1985) Studies on graft unions II. Continuous and half plasmodesmata in different regions of the graft interface. Protoplasma 126: 19–29
Marcotrigiano M, Gouin FR (1984) Experimentally synthesized plant chimeras I.In vitro recovery ofNicotiana tabacum L. chimeras from mixed callus cultures. Ann Bot 54: 503–511
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497
Steinberg C (1950) Richerche sull histogenesi dell'apice vegetative di alcune specie del genereSolanum. Nuovo G Bot Ital (NS) 57: 319–334
Sussex IM (1955) Morphogenesis inSolanum tuberosum L.: Apical structure and developmental pattern of the juvenile shoot. Phytomorphology 5: 253–273
Tilney-Bassett RAE (1986) Plant Chimeras. Arnold, London
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Dr.Josef Straub†, late Director of the Max-Planck-Institute für Züchtungsforschung at Cologne, who was the first to study the production of chimeras by callus association in 1972.
Rights and permissions
About this article
Cite this article
Binding, H., Witt, D., Monzer, J. et al. Plant cell graft chimeras obtained by co-culture of isolated protoplasts. Protoplasma 141, 64–73 (1987). https://doi.org/10.1007/BF01276789
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01276789