Summary
A polymerase chain reaction (PCR) assay was developed to detect the thymidine kinase gene of feline herpesvirus 1 (FHV-1) and to study the active and latent carrier state in a group of naturally FHV-1 infected specific pathogen free (SPF) cats. The detection limit of PCR products on ethidium bromide stained gels was 390 fg or about 3×103 copies of the FHV-1 genome. The PCR was 25% more sensitive than conventional cell culture based virus isolation techniques in detecting FHV-1 in oral/ocular swabs and 100 times more sensitive in detecting virus in cell culture supernatants. Sites of FHV-1 latency in FHV-1 carriers as determined by PCR were mainly tissues of the head, especially the trigeminal ganglia, optic nerves, olfactory bulbs and corneas. Oral fauces, salivary glands, lacrimal glands, cerebellum and conjunctiva were less consistently positive. The cerebral cortex, thymus, trachea, lung, liver, spleen, kidney, and peripheral blood mononuclear cells were consistently negative for FHV-1 genome. The distribution of FHV-1 DNA in the tissues of the head was similar whether or not corticosteroid-induced virus shedding was occurring at the time the tissues were collected. Infectious virus was never recovered from tissue homogenates regardless of the PCR status of the tissues.
Similar content being viewed by others
References
Ballagi PA, Klingeborn B, Flensburg J, Belak S (1990) Equine herpesvirus type 1: detection of viral DNA sequences in aborted fetuses with the polymerase chain reaction. Vet Microbiol 22: 373–381
Barlough JE, North TW, Oxford CL, Remington KM, Dandekar S, Ellis MN, Pedersen NC (1992) In vivo studies of feline immunodeficiency virus variants resistant to Azidothymidine (3′-azido-3′-deoxythymidine, AZT). Antiviral Res (submitted)
Boerman RH, Arnoldus EP, Raap AK, Bloem BR, Verhey M, van GG, Peters AC, van der Ploeg M (1989) Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study. J Virol Methods 25: 189–197
Cantin EM, Chen J, McNeill J, Willey DE, Openshaw H (1991) Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays. Curr Eye Res [Suppl] 10: 15–21
Collandre H, Aubin JT, Agut H, Bechet JM, Montagnier L (1991) Detection of HHV-6 by the polymerase chain reaction. J Virol Methods 31: 171–179
Crandell RA (1973) Feline viral rhinotracheitis. Adv Vet Sci Comp Med 17: 201–224
Ellis TM (1982) Feline viral rhinotracheitis virus: explant and cocultivation studies on tissues collected from persistently infected cats. Res Vet Sci 33: 270–274
Gaskell RM, Dennis PE, Goddard LE, Cocker FM, Wills JM (1984) Isolation of felid herpesvirus 1 from the trigeminal ganglia of latently infected cats. J Gen Virol 66: 391–394
Gaskell RM, Povey RC (1973) Re-excretion of feline viral rhinotracheitis virus following corticosteroid treatment. Vet Rec 93: 204–205
Gaskell RM, Povey RC (1977) Experimental induction of feline rhinotracheitis virus re-excretion in FVR recovered cats. Vet Rec 100: 128–133
Gaskell RM, Povey RC (1979) Feline viral rhinotracheitis: sites of virus replication and persistence in acutely and persistently infected cats. Res Vet Sci 27: 167–174
Grail A, Harbour DA, Chia W (1991) Restriction endonuclease mapping of the genome of feline herpesvirus type 1. Arch Virol 116: 209–220
Hickman MA, Reubel GH, Hoffmann DE, Morris JG, Rogers QR, Pedersen NC (1993) An epizootic of feline herpesvirus, type 1 in a large specific pathogen free cat colony and attempts to eradicate the infection by elimination of carriers. Lab Anim Sci (submitted)
Holbach LM, Font RL, Baehr W, Pittler SJ (1991) HSV antigens and HSV DNA in avascular and vascularized lesions of human herpes simplex keratitis. Curr Eye Res [Suppl] 10: 63–68
Hsu D, Shih LM, Castro AE, Zee YC (1990) A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction. Arch Virol 114: 259–263
Katz J, Seal B, Ridpath J (1991) Molecular diagnosis of alcelaphine herpesvirus (malignant catarrhal fever) infections by nested amplification of viral DNA in bovine blood buffy coat specimens. J Vet Diagn Invest 3: 193–198
Naeem K, Murtaugh MP, Goyal SM (1991) Tissue distribution of bovid herpesvirus-4 in inoculated rabbits and its detection by DNA hybridization and polymerase chain reaction. Arch Virol 119: 239–255
Nasisse M, Davis B, Guy J, Davidson M, Sussman W (1992) Isolation of feline herpesvirus 1 from the trigeminal ganglia of acutely and chronically infected cats. J Vet Int Med 6: 102–103
Nunberg JH, Wright DK, Cole GE, Petrovskis EA, Post LE, Compton T, Gilbert JH (1989) Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpesvirus gene homologs. J Virol 63: 3240–3249
Pedersen NC (1988) Feline infectious diseases. American Veterinary Publications, Goleta
Plummer G (1973) Isolation of herpesviruses from trigeminal ganglia of man, monkeys and cats. J Infect Dis 128: 345–348
Povey RC (1979) A review of feline rhinotracheitis (feline herpesvirus 1 infection). Comp Immun Microbiol Infect Dis 2: 373–387
Qavi HB, Green MT, SeGall GK, Hollinger FB, Lewis DE (1991) The incidence of HIV-1 and HHV-6 in corneal buttons. Curr Eye Res [Suppl] 10: 97–103
Reubel GH, George JW, Barlough JE, Grant CK, Higgins J, Pedersen NC (1992) Interaction of acute feline herpesvirus-1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats. Vet Immunol Immunpathol 35: 95–119
Rota PA, Maes RK, Ruyechan WT (1986) Physical characterization of the genome of feline herpesvirus-1. Virology 154: 168–179
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
Simmonds P, Balfe P, Peutherer JF, Ludlam CA, Bishop JO, Brown AJ (1990) Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers. J Virol 64: 864–872
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reubel, G.H., Ramos, R.A., Hickman, M.A. et al. Detection of active and latent feline herpesvirus 1 infections using the polymerase chain reaction. Archives of Virology 132, 409–420 (1993). https://doi.org/10.1007/BF01309549
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309549