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DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing

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Summary

Nucleotide sequences of bovine papillomavirus (BPV) DNA amplified by the polymerase chain reaction (PCR) from samples of equine sarcoid skin tumours were determined. All naturally occurring sarcoids (n=58 tumours from 32 horses and 2 donkeys) contained BPV-DNA. All but 3 of the genome fragments belonged to the BPV type 1 strain (BPV-1); the remaining were BPV type 2. Similar results were obtained with cutaneous bovine papillomas used as controls (n=20). One of the horses, carrying 2 sarcoids, was particularly interesting; one tumour contained BPV-1 DNA whilst the other sarcoid yielded BPV-2 DNA, suggesting that horses are not immune to super-infection. BPV-DNA was even amplified from the sarcoid samples which had yielded negative results in previous investigations when DNA isolated from the lesions was used in Southern blot hybridization with BPV probes. In addition, there was no detectable BPV-DNA in any equine or bovine tissue examined other than sarcoids or cutaneous bovine papillomas. Biopsies of normal skin surrounding lesions yielded exclusively negative results. The described nucleotide differences represent a natural genomic variation of this BPV type between geographically distant locations. The identical variations recovered from cattle and horses in Switzerland, a finding of great epidemiological interest, strongly suggest that a uniform variant of BPV-1 is one of the etiologic agents of equine sarcoid and bovine papilloma in a given region.

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References

  1. Ahola H, Stenlund J, Moreno-Lopez J, Pettersson U (1983) Sequences of bovine papillomavirus type 1 DNA — functional and evolutionary implications. Nucleic Acids Res 11: 2639–2650

    Google Scholar 

  2. Amtmann E, Müller H, Sauer G (1980) Equine connective tissue tumours contain unintegrated bovine papillomavirus DNA. J Virol 35: 962–964

    Google Scholar 

  3. Angelos JA, Marti E, Lazary S, Carmichael LE (1991) Characterization of BPV-like DNA in equine sarcoids. Arch Virol 119: 95–109

    Google Scholar 

  4. Baker JR, Leyland A (1975) Histological survey of tumours of the horse, with particular reference to those of the skin. Vet Rec 96: 419–422

    Google Scholar 

  5. Botchan M, Berg L, Reynolds J, Lusky M (1986) The bovine papillomavirus replicon. Ciba Found Symp 120: 53–67

    Google Scholar 

  6. Campo S, Moar MH, Laird HM, Jarrett WHF (1981) Molecular heterogeneity and lesion site specificity of cutaneous bovine papillomaviruses. Virology 111: 323–335

    Google Scholar 

  7. Chan SY, Bernard HU, Ong CK, Chan SP, Hofmann B, Delius H (1992) Phylogenetic analysis of 48 papillomavirus types and 28 subtypes and variants: a showcase for the molecular evolution of DNA viruses. J Virol 66: 5714–5725

    Google Scholar 

  8. Cheevers WP, Fatemi-Nainie S, Anderson LW (1986) Spontaneous expression of an endogenous retrovirus by the equine sarcoid-derived MC-1 cell line. Am J Vet Res 47: 50–52

    Google Scholar 

  9. Chen EY, Howley PM, Levinson AD, Seeburg PH (1982) The primary structure and genetic organization of the bovine papillomavirus type 1 genome. Nature 299: 529–534

    Google Scholar 

  10. Dallas PB, Flanagan JL, Nightingale BN, Morris BJ (1989) Polymerase chain reaction for fast, nonradioactive detection of high-and low-risk papillomavirus types in routine cervical specimens and in biopsies. J Med Virol 27: 105–111

    Google Scholar 

  11. Gerber H, Dubath ML, Lazary S (1988) Association between predisposition to equine sarcoid and MHC in multiple-case families. In: Powell DG (ed) Equine infectious diseases V. Univ. Press of Kentucky, Lexington, pp 272–277

    Google Scholar 

  12. Groff DE, Mitra R, Lancaster WD (1988) Unpublished

  13. Gyllensten UB, Lashkari D, Erlich HA (1990) Allelic diversification of the class II DQβ locus of the mammalian major histocompatibility complex. Proc Natl Acad Sci USA 87: 1835–1839

    Google Scholar 

  14. Han R, Breitburd F, Marche PN (1992) Linkage of regression and malignant conversion of rabbit viral papillomas to MHC class II genes. Nature 356: 66–68

    Google Scholar 

  15. Higuchi H (1989) Rapid, efficient DNA extraction for PCR from cells or blood. Amplifications 2: 2–3

    Google Scholar 

  16. Jackson C (1936) The incidence and pathology of tumours of domesticated animals in South Africa. Onderstepoort J Vet Sci Anim Indust 6: 378–385

    Google Scholar 

  17. Jarett WF (1985) The natural history of papillomaviruses. Adv Viral Oncol 5: 83–101

    Google Scholar 

  18. Kretz KA, Carson GS, O'Brien JS (1989) Direct sequencing from low-melt agarose with Sequenase®. Nucleic Acids Res 17: 5864

    Google Scholar 

  19. Lambert PF (1991) Papillomavirus DNA replication. J Virol 65: 3417–3420

    Google Scholar 

  20. Lancaster WD, Olson C, Meinke W (1977) Bovine papillomavirus. Presence of virus-specific DNA sequences in naturally occurring equine tumours. Proc Natl Acad Sci USA 74: 524–528

    Google Scholar 

  21. Lazary S, Gerber H, Glatt PA, Straub R (1985) Equine leucocyte antigens in sarcoid affected horses. Equine Vet J 17: 238–286

    Google Scholar 

  22. Meyers C, Hudson JB, Laimins LA (1992) Propagation of papillomavirus in vitro. In: Proc. 11th International Papillomavirus Workshop, Edinburgh, 1992, p 3

  23. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 17: 1215

    Google Scholar 

  24. Murray DR, Ladds PW, Campbell RSF (1978) Granulomatous and neoplastic diseases of the skin of horses. Aust Vet J 54: 338–341

    Google Scholar 

  25. Pfister H (1987) Human papillomaviruses and genital cancer. Adv Cancer Res 48: 113–147

    Google Scholar 

  26. Ragland WL, Keown GH, Gorham JR (1970) Equine sarcoid. Equine Vet J 2: 2–11

    Google Scholar 

  27. Ragland WL, Spencer GR (1968) Attempts to relate bovine papillomavirus to the cause of equine sarcoid; equidae inoculated intradermally with bovine papilloma virus. Am J Vet Res 29: 1363–1366

    Google Scholar 

  28. Ragland WL, Spencer GR (1969) Attempts to relate bovine papillomavirus to the cause of equine sarcoid; immunity to bovine papilloma virus. Am J Vet Res 30: 743–751

    Google Scholar 

  29. Reid SWJ, Smith KT (1991) The equine sarcoid: detection of papillomaviral DNA in sarcoid tumours by use of consensus primers and the polymerase chain reaction. In: Plowright W, Rossdale, PD, Wade JF (eds) Equine infectious diseases. VI. R & W Publ., Newmarket, pp 297–300

    Google Scholar 

  30. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491

    Google Scholar 

  31. Segre D, Olson C, Hoerlein AB (1955) Neutralization of bovine papillomavirus with serums from cattle and horses with experimental papillomas. Am J Vet Res 16: 517–520

    Google Scholar 

  32. Sullins KE, Roberts SM, Lavach JD, Sevrin GA, Lueker D (1986) Equine sarcoid. Equine Prac 8: 24–27

    Google Scholar 

  33. Teifke JP, Weiss E (1991) Nachweis boviner Papillomavirus-DNA in Sarkoiden des Pferdes mittels der Polymerase-Kettenreaktion (PCR). Berl Münch Tierärztl Wochenschr 6: 185–187

    Google Scholar 

  34. Trenfield K, Spradbrow PB, Vanselow B (1985) Sequences of papillomavirus DNA in equine sarcoids. Equine Vet J 17: 449–452

    Google Scholar 

  35. Voss JL (1969) Transmission of equine sarcoid. Am J Vet Res 30: 183–191

    Google Scholar 

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Authors and Affiliations

  1. Klinik für Nutztiere und Pferde, University of Berne, Berne, Switzerland

    Nathalie Otten & H. Gerber

  2. Institute for Animal Pathology, University of Berne, Berne, Switzerland

    Claudia von Tscharner

  3. Institute for Animal Breeding, Division of Immunogenetics, University of Berne, Berne, Switzerland

    S. Lazary

  4. James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA

    D. F. Antczak

Authors
  1. Nathalie Otten
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  2. Claudia von Tscharner
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  3. S. Lazary
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  4. D. F. Antczak
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  5. H. Gerber
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Otten, N., von Tscharner, C., Lazary, S. et al. DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing. Archives of Virology 132, 121–131 (1993). https://doi.org/10.1007/BF01309847

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  • DOI: https://doi.org/10.1007/BF01309847

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