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Togavirus RNA: Reversible effect of urea on genomes and absence of subgenomic viral RNA in Kunjin virus-infected cells

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Summary

Electrophoretic analyses showed that no RNase-sensitive RNA smaller than the genome was specified by the flavivirus Kunjin in infected Vero cells during the period of maximum RNA and protein synthesis. In contrast, RNA extracted from Sinbis virus-infected cells under similar conditions included the expected 42S RNA (equivalent to the genome) and the smaller 26S (interjacent) RNA. Treatment of the genome of both togaviruses with 12m urea produced a reversible (possibly conformational) change; measurement of the molecular weights of the treated RNAs by co-electrophoresis with fully denatured ribosomal RNA markers in SDS-polyacrylamide gels yielded a value of 2.1×106 if 8m urea was incorporated in the gels and 4.2×106 if urea was omitted from the gels. These results indicate that flavivirus messenger RNA is represented solely by the intact genome of m.wt. 4.2×106.

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References

  1. Arif, B. M., Faulkner, P.: Genome of Sindbis virus. J. Virol.9, 102–109 (1972).

    Google Scholar 

  2. Boulton, R. W., Westaway, E. G.: Comparisons of togaviruses: Sindbis virus (group A) and Kunjin virus (group B). Virology49, 283–289 (1972).

    Google Scholar 

  3. Boulton, R. W., Westaway, E. G.: Replication of the flavivirus Kunjin: Proteins, glycoproteins and maturation associated with cell membranes. Virology69, 416–430 (1976).

    Google Scholar 

  4. Clegg, J. C. S.: Sequential translation of capsid and membrane genes of alphavirus. Nature (Lond.)254, 454–455 (1975).

    Google Scholar 

  5. Clegg, J. C. S., Kennedy, S. I. T.: Polyadenylic acid sequences in the RNA species of cells infected with Semliki Forest virus. J. gen. Virol.22, 331–345 (1974).

    Google Scholar 

  6. Dobos, P., Faulkner, P.: Molecular weight of Sindbis virus ribonucleic acid as measured by polyacrylamide gel electrophoresis. J. Virol.6, 145–147 (1970).

    Google Scholar 

  7. Glanville, N., Ranki, M., Morser, J., Kääriänen, L., Smith, A. E.: Initiation of translation directed by 42S and 26S RNAs from Semliki Forest virusin vitro. Proc. nat. Acad. Sci. U.S.A.73, 3059–3063 (1976).

    Google Scholar 

  8. Hsu, M.-T., Kung, H.-J., Davidson, N.: An electron microscope study of Sindbis virus RNA. Cold Spring Harbour Symposia on Quantitative Biology, Vol. 38, 943–950 (1973).

    Google Scholar 

  9. Kos, K. A., Osborne, B. A., Goldsby, R. A.: Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B. J. Virol.15, 913–917 (1975).

    Google Scholar 

  10. Loening, U. E.: The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation. Biochem. J.113, 131–138 (1969).

    Google Scholar 

  11. Nishimura, C., Tsukeda, J.: Replication and synthesis of Japanese encephalitis virus ribonucleic acids in Vero cells. Japan. J. Microbiol.15, 309–316 (1971).

    Google Scholar 

  12. Reijnders, L., Sloof, P., Sival, J., Borst, P.: Gel electrophoresis of RNA under denaturing conditions. Biochim. Biophys. Acta324, 320–333 (1973).

    Google Scholar 

  13. Saborio, J. L., Pong, S.-S., Koch, G.: Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. J. mol. Biol.85, 195–211 (1974).

    Google Scholar 

  14. Stollar, V., Schlesinger, R. W., Stevens, T. M.: Studies on the nature of dengue viruses. III. RNA synthesis in cells infected with type 2 dengue virus. Virology28, 650–658 (1967).

    Google Scholar 

  15. Trent, D. W., Swensen, C. C., Qureshi, A. A.: Synthesis of Saint Louis encephalitis virus ribonucleic acid in BHK-21/13 cells. J. Virol.3, 385–394 (1969).

    Google Scholar 

  16. Westaway, E. G.: Proteins specified by group B togaviruses in mammalian cells during productive infections. Virology51, 454–465 (1973).

    Google Scholar 

  17. Westaway, E. G.: Strategy of the flavivirus genome: Evidence for multiple internal initiation of translation of proteins specified by Kunjin virus in mammalian cells. Virology80, 320–335 (1977).

    Google Scholar 

  18. Zebovitz, E., Leong, J. K. L., Doughty, S. C.: Japanese encephalitis virus replication: A procedure for the selective isolation and characterization of viral RNA species. Arch. ges. Virusforsch.38, 319–327 (1972).

    Google Scholar 

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  1. R. W. Boulton

    Present address: Department of Biochemistry, School of General Studies, Australian National University, Canberra

Authors and Affiliations

  1. Department of Microbiology, Monash University Medical School, Prahran, Melbourne, Australia

    R. W. Boulton & E. G. Westaway

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  1. R. W. Boulton
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  2. E. G. Westaway
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Boulton, R.W., Westaway, E.G. Togavirus RNA: Reversible effect of urea on genomes and absence of subgenomic viral RNA in Kunjin virus-infected cells. Archives of Virology 55, 201–208 (1977). https://doi.org/10.1007/BF01319906

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  • DOI: https://doi.org/10.1007/BF01319906

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