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High-resolution flow cytometry of nuclear DNA in higher plants

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Summary

High-resolution flow cytometry of nuclear DNA in higher plants has been performed from chopped plant tissues and plant protoplasts. A preparation and staining procedure with the DNA specific fluorochrome DAPI, successfully employed for precise flow cytometric DNA analysis of animal and human cells has been used in a slightly modified manner for the DNA analysis of plant cell material. High-resolution DNA histograms coefficients of variation about 1–1.5% have been obtained routinely from plant species with different DNA content. Staining of nuclei with DAPI in combination with the protein fluorochrome sulforhodamine 101 allows bi-parametric analysis of nuclear DNA and protein. The described simple and precise method might be very promising for the analysis of DNA in basic and applied cytogenetic investigations of plant cell research.

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Abbreviations

CV:

coefficient of variation

DAPI:

4′,6-diamidino-2-phenylindole

SR 101:

sulforhodamine 101

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Authors and Affiliations

  1. Institut für Genetik, Universität Hohenheim, Garbenstrasse 30, D-W-7000, Stuttgart 70, Federal Republic of Germany

    I. Ulrich & W. Ulrich

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  1. I. Ulrich
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  2. W. Ulrich
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Ulrich, I., Ulrich, W. High-resolution flow cytometry of nuclear DNA in higher plants. Protoplasma 165, 212–215 (1991). https://doi.org/10.1007/BF01322292

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  • DOI: https://doi.org/10.1007/BF01322292

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