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The nucleotide sequence of asinine herpesvirus 3 glycoprotein G indicates that the donkey virus is closely related to equine herpesvirus 1

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Summary

The nucleotide sequence of the glycoprotein G (gG) homologue of asinine herpesvirus 3 (AHV3), a respiratory alphaherpesvirus of donkeys, was determined. The AHV3 gG gene consists of 1233 base pairs (bp) and codes for a predicted protein of 411 amino acids. This is identical in size to the equine herpesvirus 1 (EHV1) gG gene and 6 amino acids longer than the equine herpesvirus 4 (EHV4) gG gene. The predicted amino acid sequence of AHV3 gG has characteristics of a class 1 membrane protein. The amino acid sequence of AHV3 gG shows 92% and 60% identity to EHV1 gG and EHV4 gG respectively. Two regions within the gG amino acid sequences of EHV1 and EHV4 were previously defined, an N-terminal constant region and an immunodominant highly variable region located toward the C-terminus. In the corresponding constant region of AHV3 gG there was 96% and 75% amino acid identity with EHV1 and EHV4 gGs respectively. In the variable region, there was 73% and 24% identity respectively. Phylogenetic analyses using the gG nucleotide sequences indicated that AHV3 is much closer in evolutionary distance to EHV1 than either virus is to EHV4. These findings provide additional support for the view that AHV3, or another closely related virus, may be the progenitor of EHV1 and has adapted to horses in relatively recent times.

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References

  1. Allen GP, Bryans JT (1986) Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Prog Vet Microbiol Immunol 2: 78–144

    Google Scholar 

  2. Balachandran N, Hutt-Fletcher LM (1985) Synthesis and processing of glycoprotein gG of herpes simplex virus type 2. J Virol 54: 825–832

    Google Scholar 

  3. Browning GF, Ficorilli N, Studdert MJ (1988) Asinine herpesvirus genomes: comparison with those of the equine herpesviruses. Arch Virol 101: 183–190

    Google Scholar 

  4. Crabb BS, Allen GP, Studdert MJ (1991) Characterization of the major glycoproteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3 using monoclonal antibodies. J Gen Virol 72: 2075–2082

    Google Scholar 

  5. Crabb BS, Nagesha HS, Studdert MJ (1992) Identification of equine herpesvirus 4 glycoprotein G: a type-specific, secreted glycoprotein. Virology 190: 143–154

    Google Scholar 

  6. Crabb BS, Studdert MJ (1990) Comparative studies of the proteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3: antibody response of the natural hosts. J Gen Virol 71: 2033–2041

    Google Scholar 

  7. Crabb BS, Studdert MJ (1993) Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C-termini elicit type-specific antibody responses in the natural host. J Virol 67: 6332–6338

    Google Scholar 

  8. Crabb BS, Studdert MJ (1994) Equine herpesviruses 4 (equine rhinopneumonitis virus) and 1 (equine abortion virus). Adv Virus Res 45: 153–190

    Google Scholar 

  9. Cullinane AA, Neilan J, Wilson L, Davison AJ, Allen G (1993) The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp 17/18, the homologue of herpes simplex virus glycoprotein gD. J Gen Virol 74: 1959–1964

    Google Scholar 

  10. Cullinane AA, Rixon FJ, Davison AJ (1988) Characterization of the genome of equine herpesvirus 1 subtype 2. J Gen Virol 69: 1575–1590

    Google Scholar 

  11. Felsenstein J (1989) PHYLIP-Phylogeny Interface Package (Version 3.2). Cladistics 5: 164–166

    Google Scholar 

  12. Kongsuwan K, Johnson MA, Prideaux CT, Sheppard M (1993) Identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted Mr of 32 kilodaltons. Virus Res 29: 125–140

    Google Scholar 

  13. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132

    Google Scholar 

  14. Leung-Tack P, Audonnet J-C, Riviere M (1994) The complete DNA sequence and genetic organisation of the unique short region (US) of bovine herpesvirus 1 (ST strain). Virology 199: 409–421

    Google Scholar 

  15. Mattila P, Korpela J, Tenkanen T, Pitkanen K (1991) Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase—an extremely heat stable enzyme with proof-reading activity. Nucleic Acids Res 19: 4967–4873

    Google Scholar 

  16. McGeoch DJ (1985) On the predictive recognition of signal peptide sequences. Virus Res 3: 217–286

    Google Scholar 

  17. McGeoch DJ, Dolan A, Donald S, Rixon FJ (1985) Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. J Mol Biol 181: 1–13

    Google Scholar 

  18. McGeoch DJ, Moss HW, McNab D, Frame MC (1987) DNA sequence and genetic content of the Hind III 1 region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. J. Gen Virol 68: 19–38

    Google Scholar 

  19. Nagesha HS, Crabb BS, Studdert MJ (1993) Analysis of the nucleotide sequence of five genes at the left end of the unique short region of EHV4. Arch Virol 128: 143–154

    Google Scholar 

  20. Nicolson L, Cullinane AA, Onions DE (1990) The nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus 1 glycoprotein H gene. J Gen Virol 1793–1800

  21. Nicolson L, Cullinane AA, Onions DE (1990) The nucleotide sequence of the equine herpesvirus 4 thymidine kinase gene. J Gen Virol 71: 1801–1805

    Google Scholar 

  22. Nicolson L, Onions DE (1990) The nucleotide sequence of the equine herpesvirus 4 gC gene homologue. Virology 179: 378–87

    Google Scholar 

  23. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85: 2444–2448

    Google Scholar 

  24. Rea TJ, Timmins JG, Long GW, Post LE (1985) Mapping and sequence of the gene for the pseudorabies virus glycoprotein X which accumulates in the medium of infected cells. J Virol 54: 21–29

    Google Scholar 

  25. Riggio MP, Cullinane AA, Onions DE (1989) Identification and nucleotide sequence of the glycoprotein gB gene of equine herpesvirus 4. J Virol 63: 1123–1133

    Google Scholar 

  26. Riggio MP, Onions DE (1993) DNA sequence of a gene cluster in the equine herpesvirus 4 genome which contains a newly identified herpesvirus gene encoding a membrane protein. Arch Virol 133: 171–178

    Google Scholar 

  27. Saiki RK, Gelfand DH, Stoffel S, Scarf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491

    Google Scholar 

  28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  29. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  30. Schrag JD, Prasad BVV, Rixon FJ, Chiu W (1989) Three-dimensional structure of the HSV1 nucleocapsid. Cell 56: 651–660

    Google Scholar 

  31. Studdert MJ, Crabb BS, Ficorilli N (1992) The molecular epidemiology of equine herpes virus 1 (equine abortion virus) in Australasia 1975 to 1989. Aust Vet J 69: 104–111

    Google Scholar 

  32. Su HK, Eberle R, Courtney RJ (1987) Processing of the herpes simplex virus type 2 glycoprotein gG-2 results in secretion of a 34000-Mr cleavage product. J Virol 61: 1735–1737

    Google Scholar 

  33. Telford EA, Watson MS, McBride K, Davison AJ (1992) The DNA sequence of equine herpesvirus-1. Virology 189: 304–316

    Google Scholar 

  34. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalities and weight matrix choice. Nucleic Acids Res 22: 4673–4680

    Google Scholar 

  35. Whalley JM, Robertson GR, Davison AJ (1981) Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, Bgl II, and BamHI. J Gen Virol 57: 307–323

    Google Scholar 

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Authors and Affiliations

  1. Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia

    N. Ficorilli, M. J. Studdert & B. S. Crabb

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  1. N. Ficorilli
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  2. M. J. Studdert
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  3. B. S. Crabb
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Ficorilli, N., Studdert, M.J. & Crabb, B.S. The nucleotide sequence of asinine herpesvirus 3 glycoprotein G indicates that the donkey virus is closely related to equine herpesvirus 1. Archives of Virology 140, 1653–1662 (1995). https://doi.org/10.1007/BF01322539

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  • DOI: https://doi.org/10.1007/BF01322539

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