Summary
The size of mitochondrial genomes in higher plants are known to range from 200 to 2400 kilobase pairs. However, we failed to identify cytochemically any mitochondria that contain an identifiable master mitochondrial genome. In the present experiments, we have found the giant mitochondrial nuclei which have the capacity for including the master mitochondrial genome in the young ovaries ofPelargonium zonale by use of a 4′-6-diamidino-2-phenylindole (DAPI) epifluorescence microscopy, a Technovit embedding, and a video-intensified photon counting system.
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References
Coen D, Deutsch J, Netter P, Petrochilo E, Slonimski PP (1970) Mitochondrial genetics. I. Methodology and phenomenology. In: Miller PL (ed) Control of organelle development. Symp Soc Exp Biol 24: 440–496
Kuroiwa T (1990) Application of embedding of samples in Technovit 7100 resin to observations of small amounts of DNA in cellular organelles associated with cytoplasmic inheritance. Appl Fluoresc Tech (in press)
—, Suzuki T (1980) An improved method for the demonstration of in situ chloroplast nuclei in higher plants. Cell Struct Funct 5: 195–197
Kuroiwa T, Miyamura S, Kawano S, Hizume M, Toh-e A, Miyakawa I, Sando N (1986) Cytological characterization of NOR in the bivalent ofSaccharomyces cerevisiae. Exp Cell Res 165: 199–206
Lonsdale D M, Hodge TP, Fauron CMR (1984) The physical map and organization of mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12: 9249–9261
Miyakawa I, Sando N, Kuroiwa T (1984) Fluorescence microscopic studies of mitochondrial nucleoids during meiosis and sporulation in the yeast,Saccharomyces cerevisiae. J Cell Sci 66: 21–38
Nishibayashi S, Kawano S, Kuroiwa T (1987) Light and electron microscopic observations of mitochondrial fusion in plasmodia-induced sporulation inPhysarum polycephalum. Cytologia 52: 599–614
Palmer JD, Schilds CR (1984) Tripartite structure of theBrassica campestris mitochondrial genome. Nature 307: 437–440
Sparks RB, Dale RMK (1980) Characterization of3H-labeled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet 180: 351–355
Thoman DY, Wilkie D (1968) Recombination of mitochondrial drug-resistance factors inSaccharomyces cerevisiae. Biochem Biophys Res Commun 30: 368–372
Ward BL, Anderson RS, Bendjich AJ (1981) The size of the mitochondrial genome is large and variable in a family of plants (Cucurbitaceae). Cell 25: 793–803
Wong FY, Wildman SG (1972) Simple procedure for isolation of satellite DNAs from tobacco leaves in high yield and demonstration of minicircles. Biochim Biophys Acta 259: 5–12
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Kuroiwa, T., Kuroiwa, H., Mita, T. et al. Fluorescence microscopic study of the formation of giant mitochondrial nuclei in the young ovules ofPelargonium zonale . Protoplasma 158, 191–194 (1990). https://doi.org/10.1007/BF01323132
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DOI: https://doi.org/10.1007/BF01323132