Summary
The plant growth regulator 2,4-D inhibits the incorporation of P32O4 into ATP and ADP by Chlorella to a much greater extent than it does the accumulation of inorganic phosphate by the cells.
Comparison of 2,4-D effects on P32 incorporation into nucleotide-P with those of 2,4-dinitrophenol shows that when supplied as equal concentrations of undissociated acid molecules, they are nearly equally effective.
Both 2,4-D and DNP in concentrations which inhibit the incorporation of P32 into nucleotides stimulate the uptake of oxygen by Chlorella, supporting the suggestion that they are effectively uncoupling phosphorylation.
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References
Brody, T. M., Effect of certain plant-growth substances on oxidative phosphorylation in rat-liver mitochondria. Proc. Soc. Exptl. Biol. Med.80, 533–536 (1952).
Laties, G. G., The nature of the respiratory rise in slices of chicory root. Arch. Biochem. Biophys.79, 364–377 (1959).
Simon, E. W. and Beevers, H., The effect of pH on the biological activities of weak acids and bases. I. The most usual relationship between pH and activity. New Phytologist51, 163–190 (1952).
Switzer, C. M., Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol.32, 42–44 (1957).
Wedding, R. T. and Erickson, L. C., The role of pH in the permeability of Chlorella to 2,4-D. Plant Physiol.32, 506–512 (1957).
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Wedding, R.T., Black, M.K. Uncoupling of phosphorylation in chlorella by 2,4-dichlorophenoxyacetic acid. Plant Soil 14, 242–248 (1961). https://doi.org/10.1007/BF01343855
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DOI: https://doi.org/10.1007/BF01343855