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Imaging of plant microtubules with high resolution scanning electron microscopy

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Summary

High resolution scanning electron microscopy was used to obtain images of cortical microtubules and associated structures in onion root tips. Specimens were prepared using a modified quick-freeze deep-etch technique utilising cytosolic extraction with saponin and conductive staining with osmium.

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Abbreviations

DMSO:

dimethylsulfoxide

HRSEM:

high resolution scanning electron microscope/microscopy

MTSB:

microtubule stabilising buffer

TEM:

transmission electron microscope/microscopy

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Vesk, P.A., Rayns, D.G. & Vesk, M. Imaging of plant microtubules with high resolution scanning electron microscopy. Protoplasma 182, 71–74 (1994). https://doi.org/10.1007/BF01403691

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  • DOI: https://doi.org/10.1007/BF01403691

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