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Identification of embryogenic microspores of barley (Hordeum vulgare L.) by individual selection and culture and their potential for transformation by microinjection

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Summary

In order to identify microspores, suitable for transformation via microinjection of DNA, single microspores of barley (Hordeum vulgare L.) were selected after initial preculture of anthers floating on liquid media and analysed for their development in individual culture in microdroplets of culture medium. Conditions for microculture and plant regeneration from single selected embryogenie microspores were established. The technical feasability of intranuclear microinjection was demonstrated by injecting the fluorescent dye Lucifer Yellow. All essential procedures for a transformation system of barley based on microinjection into microspores have thus been performed successfully. Further efforts to increase efficiencies of culture and microinjection procedures are necessary, however, in order to improve the suitability of this approach towards transformation of barley.

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Abbreviations

MES:

2 (N-morpholino) ethanesulfonic acid

PEG:

polyethylene glycol

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Authors and Affiliations

  1. Botanisches Institut der Universität München, Menzinger Strasse 67, D-W-8000, München 19, FRG

    Mechthild Bolik & H. U. Koop

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  1. Mechthild Bolik
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  2. H. U. Koop
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Bolik, M., Koop, H.U. Identification of embryogenic microspores of barley (Hordeum vulgare L.) by individual selection and culture and their potential for transformation by microinjection. Protoplasma 162, 61–68 (1991). https://doi.org/10.1007/BF01403902

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  • DOI: https://doi.org/10.1007/BF01403902

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