Zusammenfassung
Schnitte von arteriellen Gefäßwänden und von Thromben sowie Ausstriche von Leukocyten, die in vitro mit Kollagen Typ III inkubiert worden waren, wurden mit Hilfe der indirekten Immunfluorescenz auf die Verteilung und Anwesenheit von Kollagen Typ I, II und III untersucht. In Arterienwänden wurde Kollagen Typ III direkt subendothelial gefunden, sowie zwischen Tunica elastica interna und Adventitia. Kollagen Typ I war in der subendothelialen, der Tunica elastica interna direkt aufliegenden Schicht nicht nachweisbar. Frische Thromben enthielten gelegentlich Kollagen, jedoch nur Typ III. Dieses war mit Leukocyten assoziiert. Auch in vitro waren Leukocyten in der Lage, Kollagen Typ III zu assoziieren und/oder phagocytieren. Solches Kollagen war immunohistochemisch nachweisbar. Die Ergebnisse zeigen, daß Kollagen Typ III vermutlich eine entscheidende Rolle bei der arteriellen Thrombusbildung spielt.
Summary
Sections of arterial walls and of thrombi and smears of leukocytes previously incubated in vitro with collagen type III were examined by immunohistochemical technique for the presence of collagen types I, II and III. In arterial walls collagen type III was detected immediately underlaying the endothelial cell layer and in the tissue between tunica elastica interna and adventitia. Collagen type I was not shown in the subendothelial layer. Fresh thrombi contained occasionally collagen, but only of type III. This was associated with leukocytes. Leukocytes were capable in vitro to associate and/or phagocytose collagen type III and this could be visualized immunohistochemically. The data show that collagen type III in vivo may play a crucial role in the initiation of thrombus formation.
References
Balleisen, L., Gay, S., Marx, H., Kühn, K.: Comparative investigation on the influences of human and bovine collagen types I, II and III on the aggregation of human platelets. Klin. Wschr.53, 903–905 (1975)
Bounameaux, Y.: L'accolement des plaquettes fibres sous-endothéliales. Compt. Rend. Biol. (Paris)153, 2103 (1959)
Caen, J.P., Legrand, Y., Sultan, Y.: Platelet-collagen interactions. Thrombos. Diathes. haemorrh. (Stuttgart) Suppl.40, 181 (1970)
Epstein, E.H., Jr.: (α1(III)3) Human Skin Collagen. J. Biol. Chem.249, 3225 (1974)
Fietzek, P.P., Kühn, K.: The primary structure of collagen. International Review of Connective Tissue Res.7, in press
Gay, S., Adelmann, B.C.: Spezifische Antikörper gegen Kollagentyp I und Kollagentyp II: Immunfluoreszenzoptische Differenzierung und selektive Zytolyse von Typ I-bzw. Typ II-synthetisierenden Bindegewebszellen. Zschr. Immun. forsch.147, 315 (1974)
Gay, S., Adelmann, B.C., Remberger, K.: Identification by immunofluorescence of collagen type I, II and III in tissue. In preparation
Hahn, E., Timpl, R., Miller, E.J.: The production of specific antibodies to native collagens with the chain composition (αl(I)3), (αl(II)3) and (αl(I)2α2). J. Immunol.113, 421 (1974)
Rauterberg, J.: Characterization of a genetic variant of collagen (Type III) from calf aorta. IV. Europ. Symp. Connect. Tiss. Res. Padua 106 (1974)
Stemerman, M.B.: Platelet interaction with intimal connective tissue, in: Baldini, M.G. and Ebbe, S.: Platelets: Production, function transfusion and storage; p. 157, Grune + Stratton, Inc. New York, San Francisco, London 1974
Wick, G., Furthmayr, H., Timpl, R.: Purified antibodies to collagen: An immunofluorescence study of their reaction with tissue collagen. Int. Arch. Allergy, submitted
Wilner, G.D., Nossel, H.L., LeRoy, E.C.: Aggregation of platelets by collagen. J. Clin. Invest.47, 2616 (1968)
Zucker, M.B., Borrelli, J.: Platelet clumping produced by connective tissue suspensions and by collagen. Proc. Soc. Exp. Biol. Med.109, 779 (1962)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gay, S., Balleisen, L., Remberger, K. et al. Immunohistochemical evidence for the presence of collagen type III in human arterial walls, arterial thrombi, and in leukocytes, incubated with collagen in vitro. Klin Wochenschr 53, 899–902 (1975). https://doi.org/10.1007/BF01468981
Issue Date:
DOI: https://doi.org/10.1007/BF01468981