Abstract
Somatic cell fusion was used to study the genetic defect in Menkes' disease and in the brindled mouse mutant. Eight hybrids derived from fusion of Menkes' fibroblasts with HeLa cells and 11 hybrids between a brindled mouse fetal cell line and a mouse A9 cell were studied. The hybrids showed copper isotope uptake intermediate between the mutant and nonmutant lines and copper isotope efflux similar to that of the nonmutant parent. These results indicate that complementation studies between the mutants should be possible.
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Literature cited
Goka, T.J., Stevenson, R.E., Hefferan, P.M., and Howell, R.R. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:604–606.
Horn, N. (1976). Lancet1:1156–1158.
Beratis, N.G., Price, P., LaBadie, G., and Hirschhorn, K. (1978).Pediatr. Res. 12:699–702.
Camakaris, J., Mann, J.R., and Danks, D.M. (1979).Biochem. J. 180:597–604.
Camakaris, J., Danks, D.M., Ackland, L., Cartwright, E., Borger, P., and Cotton, R.G.H. (1980).Biochem. Genet. 18:117–131.
Chan, W., Garnica, A.D., and Rennert, O.W. (1978).Clin. Chim. Acta 88:495–507.
Povey, S., Hopkinson, D.A., and Harris, H. (1976).Nature 264:60–63.
Maio, J.J., and De Carli, L.L. (1962).Nature 196:600–601.
Littlefield, J.W. (1964).Nature 203:1142–1144.
Davidson, R.L., and Gerald, P.S. (1976).Somat. Cell Genet. 2:165–176.
Littlefield, J.W. (1964).Science 145:709–710.
Seabright, M. (1971).Lancet 2:971–972.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951).J. Biol. Chem. 193:265–275.
Corsaro, C.M., and Migeon, B.R. (1977).Nature 268:737–739.
Corsaro, C.M., and Migeon, B.R. (1978).Somat. Cell Genet. 4:541–551.
Watson, B., Gormley, I.P., Gardiner, S.E., Evans, H.J., and Harris, H. (1972).Exp. Cell Res. 75:401–409.
Bakay, B., Croce, C.M., Koprowski, H., and Nyhan, W.L. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:1998–2002.
Croce, C.M., Bakay, B., Nyhan, W.L., and Koprowski, H. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:2590–2594.
Bakay, B., Graf, M., Carey, S., Nissinen, E., and Nyhan, W.L. (1978).Biochem. Genet. 16:227–237.
Davidson, R.L., and Ephrussi, B. (1965).Nature 205:1170–1171.
Green, M.C. (ed.) (1981).Genetic Variants and Strains of the Laboratory Mouse (Verlag, Stuttgart).
Hunt, D.M. (1974).Nature 249:852–854.
Danks, D.M. (1977).Inorg. Perspect. Biol. Med. 1:73–100.
Danks, D.M., and Camakaris, J. (1983).Adv. Hum. Genet. 13:149–216.
Procopis, P., Camakaris, J., and Danks, D.M. (1981).J. Pediatr. 98:97–99.
Kuivaniemi, H., Peltonen, L., Palotie, A., Kaitila, I., and Kivirikko, K.I. (1982).J. Clin. Invest. 69:730–733.
Danks, D.M., Cartwright, E., Stevens, B.J., and Townley, R.R.W. (1973).Science 179:1140–1141.
LaBadie, G.U., Beratis, N.G., and Hirschhorn, K. (1983).Pediatr. Res. 17:213A.
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This work was supported by a grant from the National Health and Medical Research Council of Australia.
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Brown, R.M., Camakaris, J. & Danks, D.M. Observations on the Menkes' and brindled mouse phenotypes in cell hybrids. Somat Cell Mol Genet 10, 321–330 (1984). https://doi.org/10.1007/BF01535628
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DOI: https://doi.org/10.1007/BF01535628