Abstract
Human phosphofructokinase (PFK; EC 2.7.1.11) is under the control of three structural loci which encode muscle-type (M), liver-type (L), and platelet-type (P) subunits; human diploid fibroblasts and leukocytes express all three loci. In order to assign human PFKMlocus to a specific chromosome we have analyzed human × Chinese hamster somatic cell hybrids for the expression of human M subunits, using an anti-human M subunit-specific mouse monoclonal antibody. In 18 of 19 hybrids studied, the expression of the PFKMlocus segregated concordantly with the presence of chromosome 1 (discordancy rate 0.05) as indicated by chromosome and isozyme marker analysis. The discordancy rates for all the other chromosomes were 0.32 or greater, indicating that the PFKMlocus is on chromosome 1. For the regional mapping of PFKM,eight hybrids were studied that contained one of five distinct regions of chromosome 1. These results further localize the human PFKMlocus to region cen→q32 of chromosome 1.
Similar content being viewed by others
Literature cited
Bloxham, D.P., and Lardy, H.A. (1973). Phosphofructokinase. InThe Enzymes, Vol. 8A, (ed.) Boyer, P.D. (Academic Press, New York), pp. 239–278.
Tarui, S., Okuno, G., Ikura, Y., Tanaka, T., Suda, M., and Nishikawa, M. (1965).Biochem. Biophys. Res. Commun. 19:517–523.
Layzer, R.B., Rowland, L.P., and Ranney, H.M. (1967).Arch. Neurol. 17:512–523.
Vora, S., Corash, L., Engel, W.K., Durham, S., Seaman, C., and Piomelli, S. (1980).Blood 55:628–635.
Baikie, A.F., Luder, P.B., de Grouchy, G.C. and Pitt, D.B. (1965).Lancet 1:412–414.
Vora, S., Seaman, C., Durham, S., and Piomelli, S. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:62–68.
Vora, S. (1981).Blood 57:724–732.
Vora, S., Wims, A.L., Durham, S., and Morrison, S.L. (1981).Blood 58:823–829.
Vora, S., and Francke, U. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:3738–3742.
Vora, S., Durham, S., de Martinville, B., Francke, U. (1981). Human Gene Mapping 6 Oslo Conference.Cytogenet. Cell Genet (in press).
Francke, U., and Pellegrino, M.A. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:1147–1151.
Sanders-Haigh, L., Anderson, W.F., and Francke, U. (1980).Nature 283:683–686.
Francke, U., and Francke, B. (1981).Somat. Cell. Genet. 7:171–192.
Francke, U., Busby, N., Shaw, D., Hansen, S., and Brown, M.G. (1976).Somat. Cell. Genet. 2:27–40.
Francke, U. (1975).Cytogenet. Cell. Genet. 14:308–312.
Francke, U., and Oliver, N. (1978).Hum. Genet. 45:137–165.
Meera Khan, P. (1971).Arch. Biochem. Biophys. 145:470–483.
Francke, U. (1981).Cytogenet. Cell Genet. 31:25–32.
Human Gene Mapping 5 (1979).Cytogenet. Cell Genet. 25:1–236.
Dunaway, G.A., Morris, H.P., and Weber, G. (1974).Cancer Res. 34:2209–2216.
Tsai, M.Y., and Kemp, R.G. (1973).J. Biol. Chem. 248:785–792.
Tsai, M.Y., and Kemp, R.G. (1974).J. Biol. Chem. 249:6590–6596.
Markert, C.L. (1977). InIsozymes: Current Topics in Biological and Medical Research, Vol. 1, (eds.) Rattazzi, M.C., Scandalios, J. G., and Whitt, G.S. (Liss, New York) p. 6.
Weil, D., Cottreau, D., van Cong, N., Rebourcet, R., Foubert, C., Gross, M-S., Dreyfus, J-C., and Kahn, A. (1980).Ann. Hum. Genet.,44:11–16.
Ohno, S. (1970).Evolution by Gene Duplication, (Springer-Verlag, New York), pp. 89–106.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vora, S., Durham, S., de Martinville, B. et al. Assignment of the human gene for muscle-type phosphofructokinase (PFKM) to chromosome 1 (region cen→q32) using somatic cell hybrids and monoclonal anti-M antibody. Somat Cell Mol Genet 8, 95–104 (1982). https://doi.org/10.1007/BF01538653
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01538653