Abstract
Hybrid cells derived from whole-cell fusions of replicating phase-II normal fibroblast cells (WI-38s) with SV40 transformed WI-38 fibroblast cells (CL-1s) demonstrated that the majority of the hybrid experimental cells still maintained a finite life-span. Approximately 2% demonstrated sustained and possibly indefinite replication. Experimental binucleate cells and subsequent hybrid synkaryons were also formed by fusing CL-1 karyoplasts into phase-II WI-38 replicating normal fibroblasts. In addition, viable cells were constructed from WI-38 fibroblast cytoplasts with CL-1 karyoplasts. Sustained replication was not observed in these crosses.
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Muggleton-Harris, A.L., DeSimone, D.W. Replicative potentials of various fusion products between WI-38 and SV40 transformed WI-38 cells and their components. Somat Cell Mol Genet 6, 689–698 (1980). https://doi.org/10.1007/BF01538968
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DOI: https://doi.org/10.1007/BF01538968