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Regional mapping of human genes for hexosaminidase B and diphtheria toxin sensitivity on chromosome 5 using mouse × human hybrid cells

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Somatic Cell Genetics

Abstract

Mouse 3T3 (TK−) cells were fused to human leukocytes containing a balanced translocation [ins(3;5) (q27;q13q15)] in which part of the long arm of a chromosome 5 has been inserted into the long arm of a chromosome 3. Two independent, primary hybrid clones (XVI-10C; XVI-18A) retained the deleted chromosome 5 [del(5) (q13q15)] translocation product and were informative for regional mapping on chromosome 5 of genes involved in expression of hexosaminidase B (HEX B ) and diphtheria toxin sensitivity (DTS). Both XVI-10C and XVI-18A clones were sensitive to diphtheria toxin. Toxin-resistant derivatives of these clones (XVI-10C DTR; XVI-18A DTR) were analyzed for chromosome content and expression of Hex B activity, as were XVI-10C and XVI-18A cells which had not been exposed to diphtheria toxin. The results of this study provide evidence for localization ofDTS to region 5q15→5qter on the long arm of chromosome 5, and localization ofHEX B to region 5pter→5q13.

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Authors and Affiliations

  1. Department of Pediatrics M-009, School of Medicine, University of California, 92093, San Diego La Jolla, California

    Donna L. George & Uta Francke

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  1. Donna L. George
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  2. Uta Francke
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George, D.L., Francke, U. Regional mapping of human genes for hexosaminidase B and diphtheria toxin sensitivity on chromosome 5 using mouse × human hybrid cells. Somat Cell Mol Genet 3, 629–638 (1977). https://doi.org/10.1007/BF01539070

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  • DOI: https://doi.org/10.1007/BF01539070

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