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Chromosome assignment of genes encoding theα andΒ subunits of glycoprotein hormones in man and mouse

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Somatic Cell Genetics

Abstract

The chromosomal locations of the genes for the common α subunit of the glycoprotein hormones and the Β subunit of chorionic gonadotropin in humans and mice have been determined by restriction enzyme analysis of DNA isolated from somatic cell hybrids. The CGα gene (CGA), detected as a 15-kb BamHI fragment in human DNA by hybridization to CGα cDNA, segregated with the chromosome 6 enzyme markers ME1 (malic enzyme, soluble) and SOD2 (superoxide dismutase, mitchondrial) and an intact chromosome 6 in human-rodent hybrids. Cell hybrids containing portions of chromosome 6 allowed the localization of CGA to the q12 → q21 region. The >30- and 6.5-kb BamHI CGB fragments hybridizing to human CGΒ cDNA segregated concordantly with the human chromosome 19 marker enzymes PEPD (peptidase D) and GPI (glucose phosphate isomerase) and a normal chromosome 19 in karyotyped hybrids. A KpnI-HindIII digest of cell hybrid DNAs indicated that the multiple copies of the CGΒ gene are all located on human chromosome 19. In the mouse, the α subunit gene, detected by a mouse thyrotropin (TSH) α subunit probe, and the CGΒ-like sequences (CGΒ-LHΒ), detected by the human CGΒ cDNA probe, are on chromosomes 4 and 7, respectively.

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Authors and Affiliations

  1. Department of Human Genetics, Roswell Park Memorial Institute, New York State Department of Health, 14263, Buffalo, New York

    Susan L. Naylor & Alan Y. Sakaguchi

  2. Laboratory of Molecular Endocrinology, Massachusetts General Hospital, 02114, Boston, Massachusetts

    William W. Chin

  3. Howard Hughes Medical Institute Laboratories, Harvard Medical School, 02114, Boston, Massachusetts

    William W. Chin

  4. Department of Molecular Biology, Massachusetts General Hospital, 02114, Boston, Massachusetts

    Howard M. Goodman

  5. Department of Genetics, Harvard Medical School, 02114, Boston, Massachusetts

    Howard M. Goodman

  6. Biology Division, Oak Ridge National Laboratories, 37830, Oak Ridge, Tennessee

    Peter A. Lalley

  7. Institut für Human genetik, Westfälische Wilhelms-Universität, Münster, West Germany

    Karl-Heinz Grzeschik

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  1. Susan L. Naylor
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  2. William W. Chin
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  3. Howard M. Goodman
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  4. Peter A. Lalley
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  5. Karl-Heinz Grzeschik
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  6. Alan Y. Sakaguchi
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Naylor, S.L., Chin, W.W., Goodman, H.M. et al. Chromosome assignment of genes encoding theα andΒ subunits of glycoprotein hormones in man and mouse. Somat Cell Mol Genet 9, 757–770 (1983). https://doi.org/10.1007/BF01539478

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  • DOI: https://doi.org/10.1007/BF01539478

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