Abstract
In order to map human PGKsequences, DNA was prepared from 55 human-mouse somatic cell lines. The DNA was digested to completion with HindIII and Southern filters prepared. These filters were hybridized at high stringency conditions to a human PGKcDNA. Mouse and human X-linked and autosomal bands were distinguished and, in addition to known X-linked sequences, two autosomal PGKsequences were mapped: a 1-kb band to chromosome 19 and a 5-kb band to chromosome 6. The PGKcDNA probe was also hybridized to flow-sorted chromosomes confirming the presence of PGK sequences on the X chromosome and chromosomes 6 and 19.
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Literature cited
Vandeberg, J.L., Cooper, D.W., and Close, P.J. (1973).Nature (London), New Biol. 243:48–50.
Gartler, S.M., and Riggs, A.D. (1983).Annu. Rev. Genet. 17:155–190.
Goldman, M.A., Gartler, S.M., Keitges, E.A., and Riley, D.E. (1985). InHuman Genes and Disease, Vol. 8, Horizons in Biochemistry and Biophysics, (ed.) Blasi, F. (Wiley & Sons, New York), (in press).
Singer-Sam, J., Keith, D.H., Tani, K., Simmer, R.L., Shively, L., Lindsay, S., Yoshida, A., and Riggs, A.D. (1984).Gene 32:409–417.
Michelson, A.M., Bruns, G.A.P., Morton, C.C., and Orkin, S.H. (1985).J. Biol. Chem. 260:6982–6992.
Szabo, P., Grzeschik, K., and Siniscalco, M. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:3167–3169.
Michelson, A.M., Blake, C.C.F., Evans, S.T., and Orkin, S.H. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:6965–6969.
Eicher, E.M., Cheny, M., and Flaherty, L. (1978).Mol. Gen. Genet. 158:225–228.
Willard, H.F., Gon, S.J., Holmes, M.T., and Munroe, D.L. (1985).Hum. Genet. 71:138–143.
Riley, D.E., Canfield, T.K., and Gartler, S.M. (1983).Nucleic Acids Res. 12:1829–1845.
Shows, T.B. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:348–352.
Shows, T.B., Sakaguchi, A.Y., and Naylor, S.L. (1982). InAdvances in Human Genetics, Vol. 12, (eds.) Harris, H., and Hirschhorn, K. (Plenum Press, New York), pp. 341–452.
Shows, T.B., Eddy, R., Haley, L., Byers, M., Henry, M., Fujita, T., Matsui, H., and Taniguchi, T. (1984).Somat. Cell. Mol. Genet 10:315–318.
Naylor, S.L., Sakaguchi, A.Y., Shows, T.B., Law, M.L., Goeddel, D.V., and Gray, P.W. (1983).J. Exp. Med. 57:1020–1027.
Southern, E.M. (1975).J. Mol. Biol. 98:503–517.
Lebo, R.V., Gorin, F., Fletterick, R.J., Kao, F.T., Cheung, M.C., Bruce, B.O., and Kan, Y.W. (1984).Science 225:57–59.
Lebo, R.V., and Batian, A.M. (1982).Cytometry 3:213–219.
Michelson, A.M., Markman, A.F., and Orkin, S.H. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:472–476.
Singer-Sam, J., Simmer, R.L., Keith, D.H., Shively, L., Tiplitz, M., Itakura, K., Gartler, S.M., and Riggs, A.D. (1983).Proc. Natl. Acad. Sci. U.S.A. 80: 802–806.
de la Chapelle, A., and Miller, O.J. (1979).Cytogenet. Cell Genet. 25:47–58.
Tani, K., Singer-Sam, J., Munns, M., and Yoshida, A. (1985).Gene 35:11–18.
Chance, P.F., Kurachi, K., Ropers, H., Wieacker, P., and Gartler, S.M. (1983).Am. J. Hum. Genet. 35:187A.
Shows, T.B., McAlpine, P.J., and Miller, R.L. (1984).Cytogenet. Cell Genet. 37:340–393.
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Gartler, S.M., Riley, D.E., Lebo, R.V. et al. Mapping of human autosomal phosphoglycerate kinase sequence to chromosome 19. Somat Cell Mol Genet 12, 395–401 (1986). https://doi.org/10.1007/BF01570734
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DOI: https://doi.org/10.1007/BF01570734