The quantitative ultrastructure of the pea shoot apex in relation to leaf initiation

Summary

The ultrastructure of the pea shoot apical meristem was examined quantitatively in longitudinal sections. Photographs were taken at eleven defined positions in the apex, at six developmental stages within a single plastochron. The only change in ultrastructure during the period of a single plastochron was the increase in the proportion of plastids with starch in the central regions of the apex and in the young leaf axils. This increase occurred midway in time between the emergence of successive leaves, at precisely the time that the orientation of growth changes in the region where a new leaf is to emerge. There were quantitative changes in ultrastructure associated with cell differentiation. In the sequence of cell development from the summit of the apex (central zone) to the incipient pith, cell enlargement was accompanied by an increase in the volume of endoplasmic reticulum, dictyosomes, microbodies and vacuoles per cell, an increase in the number of mitochondria, microbodies and vacuoles per cell, and an increase in the volume, but not the number, of plastids per cell. In the sequence of axillary development (before the axillary bud begins to grow) the number of mitochondria per cell decreased as cell volume decreased but the number of plastids per cell remained constant. The number of plastids per cell increased only in the developmental sequence leading to leaf development, in which the number of mitochondria and dictyosomes per cell also increased. There appeared to be no features of ultrastructure, qualitative or quantitative, which could be correlated with the different rates of cell division in different regions of the meristem. The differences in ultrastructure throughout the apex were mainly quantitative and seemed to be associated with cellular differentiation rather than with the plastochronic functioning of the apex during leaf initiation.