Abstract
Phospholipids extracted fromSaccharomyces cerevisiae at different stages of development after glucose repression contain three major fatty acids: palmitic, palmitoleic and oleic. The ratio palmitic: palmitoleic strongly decreases beginning at the 6th hour of growth.
To test the effect of fatty acid composition and in particular of unsaturation on succinoxidase activity, all these phospholipids, phospholipids from commercial yeast, and Asolectin were incubated with lipid-depleted yeast mitochondria. The amount of P bound was not much different for the various phospholipids; succinoxidase activity was restored best by Asolectin; the least effective reactivation was given by phospholipids from yeast at the middle stages of growth. There are not great differences between the various phospholipids and there is no correlation with unsaturation. If we compare the pattern of appearance of respiration during morphogenesis of yeast mitochondria with the pattern of the capability of the phospholipids from cells at different stages of mitochondrial morphogenesis to restore activity of lipid-depleted yeast mitochondria, we find no correlation. The results of this investigation are consistent with the idea that changes in phospholipids and changes in enzyme activities are not linked by a causal relation.
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A. Castelli, G. Parenti-Castelli, E. Bertoli, and G. Lenaz,Ital. J. Biochem.,18 (1969) 35.
G. Lenaz, G. P. Littarru, and A. Castelli,FEBS Letters,2, (1969) 198.
P. G. Wallace and A. W. Linnane,Nature 201 (1964) 1191.
P. G. Wallace, M. Huang, and A. W. Linnane,J. Cell Biol.,37 (1968) 207.
D. Jollow, G. M. Kellerman, and A. W. Linnane,J. Cell Biol. 37 (1968) 221.
H. B. Lukins, D. Jollow, P. G. Wallace, and A. W. Linnane,Aust. J. exp. Biol. med. Sci.,46 (1968) 651.
F. Paltauf and G. Schatz,Biochemistry,8, (1969) 335.
G. Lenaz, A. M. Sechi, G. Parenti-Castelli, L. Masotti, A. Castelli, G. P. Littarru, and E. Bertoli, inAutonomy and Biogenesis of Mitochondria and Chloroplasts, N. K. Boardman, A. W. Linnane, and R. M. Smillie (eds.), p. 119, 1970.
A. Castelli, G. Barbaresi, and E. Bertoli,Ital. J. Biochem. 18 (1969) 91.
L. Kovac, J. Subik, G. Russ, and K. Kollark,Biochim. Biophys. Acta,144 (1967) 94.
D. E. Green and A. Tzagoloff,J. Lipid Res.,7 (1966) 587.
G. Lenaz, A. M. Sechi, L. Masotti, and G. Parenti-Castelli,Arch. Biochem. Biophys.,141 (1970) 79.
G. Lenaz, A. M. Sechi, G. Parenti-Castelli, and L. Masotti,Arch. Biochem. Biophys.,141 (1970) 89.
J. Cabo-Soler, A. M. Sechi, G. Parenti-Castelli, and G. Lenaz,J. Bioenergetics, in press.
J. S. O'Brien,Science,147 (1965) 1099.
G. Schatz,Methods Enzymol.,10, (1967) 199.
S. Fleischer and B. Fleischer,Methods Enzymol.,10 (1967) 406.
J. Folch, M. Lees, and G. H. Sloane-Stanley,J. Biol. Chem.,226 (1957) 497.
P. A. Marks, A. Gellhorn, and C. Kidson,J. Biol. Chem.,235 (1960) 2579.
A. T. James,Methods Biochem. Anal.,8 (1960) 1.
R. L. Lester and S. Fleischer,Biochim. Biophys. Acta 47 (1961) 358.
A. G. Gornall, C. J. Bardawill, and M. M. David,J. Biol. Chem.,177 (1949) 751.
P. V. Marinetti,J. Lipid Res.,3 (1962) 1.
S. Fleischer, G. Brierley, H. Klouwen, and D. B. Slautterback,J. Biol. Chem.,237 (1962) 3264.
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Bertoli, E., Barbaresi, G., Castelli, A. et al. Effect of phospholipids from Saccharomyces cerevisiae at different stages of development on restoration of succinooxidase activity in lipid-depleted mitochondria. J Bioenerg Biomembr 2, 135–140 (1971). https://doi.org/10.1007/BF01648908
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DOI: https://doi.org/10.1007/BF01648908