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The effect of glutaraldehyde on light-induced H+ changes, electron transport, and phosphorylation in pea chloroplasts

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Summary

This investigation has shown the following hierarchy of chloroplast reactions which were susceptible to increasing glutaraldehyde concentration given for 6 min at 0° C.

  1. (1)

    Cyclic and non-cyclic photophosphorylation was completely inhibited by 100 mM glutaraldehyde.

  2. (2)

    Reduction in the rate of H+ uptake, and increase in the rate of H+ efflux occurred to give a ratio uptake/loss of unity with 100 mM glutaraldehyde.

  3. (3)

    Volume fixation occurred at 200 mM glutaraldehyde.

  4. (4)

    Fixed chloroplasts were resistant to stimulation of H+ and electron transport by weak acid anions and uncouplers.

  5. (5)

    Light-induced electron-transport activity progressively decreased. About 35% inhibition of the basal (non-phosphorylating) rate and 60% inhibition of the uncoupled rate occurred on fixation.

  6. (6)

    The extent of the light-induced H+ changes were reduced only by about 10% upon fixation.

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References

  1. R. B. Park, J. Kelly, S. Drury, and K. Sauer,Proc. Nat. Acad. Sci.,55 (1966) 1056.

    Google Scholar 

  2. L. Packer, J. M. Allen, and M. Starks,Arch. Biochem. Biophys.,128, (1968) 142.

    Google Scholar 

  3. U. W. Hallier and R. B. Park,Plant Physiol.,44 (1969) 535.

    Google Scholar 

  4. U. W. Hallier and R. B. Park,Plant Physiol.,44 (1969) 544.

    Google Scholar 

  5. K. Utsumi, and L. Packer,Arch. Biochem. Biophys.,121 (1967) 633.

    Google Scholar 

  6. T. Treffry,Planta,85 (1969) 376.

    Google Scholar 

  7. B. Chance and G. R. Williams,J. Biol. Chem.,217 (1955) 383.

    Google Scholar 

  8. K. R. West and J. T. Wiskich,Biochem. J.,109 (1968) 527.

    Google Scholar 

  9. D. I. Arnon,Plant Physiol.,24 (1949) 1.

    Google Scholar 

  10. J. West and R. Hill,Plant Physiol.,42 (1967) 819.

    Google Scholar 

  11. M. Nishimura, T. Ito, and B. Chance,Biochim. Biophys. Acta,59 (1962) 177.

    Google Scholar 

  12. M. Schwartz,Nature,219 (1968) 915.

    Google Scholar 

  13. B. Hagihara and H. A. Lardy,J. Biol. Chem.,235 (1960) 889.

    Google Scholar 

  14. A. A. Horton and L. Packer,Arch. Biochem. Biophys.,128 (1968) 820.

    Google Scholar 

  15. R. A. Dilley and A. Rothstein,Biochim. Biophys. Acta,135 (1967) 427.

    Google Scholar 

  16. S. Murakami and L. Packer,J. Cell Biol., in press.

  17. P. Mitchell in:Theoretical and Experimental Biophysics, Vol. II A. Cole (Ed.), Marcel Dekker, New York, 1969, pp. 159–216.

    Google Scholar 

  18. W. Junge and H. T. Witt,Zeitschr. f. Naturforsch.,23b (1968) 244.

    Google Scholar 

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Author information

Author notes

  1. Janet West (Broodbank Fellow)

    Present address: Department of Biochemistry, ARC Institute of Animal Physiology, Babraham, Cambridge, England

  2. Lester Packer

    Present address: Department of Physiology, University of California, 94720, Berkeley, California

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Cambridge, England

    Janet West (Broodbank Fellow) & Lester Packer

  2. Department of Biochemistry, ARC Institute of Animal Physiology, Babraham, Cambridge, England

    Lester Packer

Authors
  1. Janet West
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  2. Lester Packer
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West, J., Packer, L. The effect of glutaraldehyde on light-induced H+ changes, electron transport, and phosphorylation in pea chloroplasts. J Bioenerg Biomembr 1, 405–412 (1970). https://doi.org/10.1007/BF01654577

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  • DOI: https://doi.org/10.1007/BF01654577

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