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Correlation of anti-cytoskeleton antibody activities in synovial fluid with interleukin-6 in patients with osteoarthritis and inflammatory joint disease

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Summary

Synovial fluids and sera from patients with rheumatoid arthritis, psoriatic arthritis, yersinia arthritis, Behçet's syndrome, Crohn's disease, and osteoarthritis were tested for antinuclear antibodies and antibodies to five cytoskeletal components in sensitive enzyme-linked immunosorbent assay (ELISA) systems and for IL-6 concentrations in a proliferation assay (IL-6 dependent hybridoma cell line B13.29, subclone B9). Statistically significant correlations between antibody activities and IL-6 levels were found for vimentin antibodies (r= 0.56; p<0.05) and actin antibodies (r= 0.44;p<0.05). In patients with chronic and active disease like rheumatoid arthritis and psoriatic arthritis, optical densities measured by vimentin- and actin-ELISA were significantly different from those measured in patients with osteoarthritis. To date only a few reports exist concerning the incidence of antibodies in synovial fluids. We have shown to our knowledge for the first time that IL-6 seems to induce synovial fluid antibody activities restricted to cytoskeletal components of synoviocytes (i.e., vimentin and actin). Synovial fluid antibody activities against vimentin and actin appear to be markers of activity in patients with inflammatory joint disease.

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Abbreviations

ELISA:

enzyme-linked immunosorbent assay

ANA:

antinuclear antibodies

ACA:

antibodies against cytoplasmic components

RA:

rheumatoid arthritis

OA:

osteoarthritis

SF:

synovial fluid

RF:

rheumatoid factors

FCS:

fetal calf serum

IFT:

immunofluorescence microscopy

PBS:

phosphate buffered saline

BSA:

bovine serum albumin

OD:

optical density

SLE:

systemic lupus erythematosus

IL-6:

interleukin-6

HGF:

hybridoma growth factor

BSF-2:

B-cell-stimulatory factor

DMEM:

Dulbecco's modified Eagles Medium

References

  1. Aarden LA, De Groot ER, Schaap OL (1987) Production of hybridoma growth factor by human monocytes. Eur J Immunol 17:1411–1416

    Google Scholar 

  2. Abrahamsen TG, Froland SS, Natvig JB (1975) Elution and characterization of lymphocytes from rheumatoid inflammatory tissue. Scand J Immunol 4:823–830

    Google Scholar 

  3. Al-Balaghi S, Strom H, Moller E (1984) B cell differentiation factor in synovial fluid of patients with rheumatoid arthritis. Immunol Rev 78:7–23

    Google Scholar 

  4. Clague RB, Moore LJ (1984) IgG and IgM antibody to native type II collagen in rheumatoid arthritis serum and synovial fluid. Evidence for the presence of collagen-anticollagen immune complexes in synovial fluid. Arthritis Rheum 27:1370–1377

    Google Scholar 

  5. Egeland T, Lea T, Saari G, Mellbye OJ, Natvig JB (1982) Quantitation of cells secreting rheumatoid factors of IgG, IgA and IgM class after elution from rheumatoid synovial tissue. Arthritis Rheum 25:1445–1450

    Google Scholar 

  6. Fay AC, Trudgett A, McCrea JD (1958) Detection and partial characterization of human B cell colony stimulating activity in synovial fluids of patients with rheumatoid arthritis. Clin Exp Immunol 60:316–322

    Google Scholar 

  7. Hermann E, Fleischer B, Mayet WJ, Poralla T, Meyer zum Büschenfelde KH (1989) Correlation of synovial fluid interleukin 6 (IL-6) activities with IgG concentrations in patients with inflammatory joint disease and osteoarthritis. Clin Exp Rheumatol 7:411–414

    Google Scholar 

  8. Hirano T, Yasukawa K, Harada H, Taga T, Watanabe Y (1986) Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324:73–76

    Google Scholar 

  9. Hollywell C, Morris CJ, Farr M, Walton KW (1983) Ultra-structure of synovial changes in rheumatoid disease and in seronegative inflammatory arthropathies. Virchows Arch (Pathol Anat) 400:345–355

    Google Scholar 

  10. Houssiau FA, Devogelaer JP, van Damme J, Nagant de Deuxchaisnes C, van Snick J (1988) Interleukin-6 in synovial fluid and serum of patients with rheumatoid arthritis and other inflammatory arthritides. Arthritis Rheum 31:784–787

    Google Scholar 

  11. Kirstein H, Hjarvard K, Hansen TM (1989) Antikeratin antibodies in synovial fluid in rheumatoid arthritis. APMIS 97:185–189

    Google Scholar 

  12. Kitani A, Hara M, Hirose T (1987) Synovial fluids from patients with rheumatoid arthritis possess B cell differentiation activity contributing to synthesis of rheumatoid factor in vitro. J Rheumatol 14:873–879

    Google Scholar 

  13. Koopman WJ, Schrohenloher RE, Crago SS, Spalding DM, Mestecky J (1985) IgA rheumatoid factor synthesis by dissociated synovial cells. Characterization and relationship to IgM rheumatoid factor synthesis. Arthritis Rheum 28:1219–1227

    Google Scholar 

  14. Mayet WJ, Hess G, Gerken G, Rossol S, Voth R, Manns M, Meyer zum Büschenfelde KH (1989) Treatment of chronic type B hepatitis with recombinant alpha-interferon induces autoantibodies not specific for autoimmune chronic hepatitis. Hepatology 10:24–28

    Google Scholar 

  15. Meyer O, Semmache M, Cyna J, Mitrovic D, Ryckewaert A (1983) Les anticorps anticollagene. Detection au cours de la polyarthrite rhumatoide, de la polychondrite chronique atrophiante et de divers rhumatismes inflammatoires chroniques. Rev Rhum Mal Osteoartic 50:493–499

    Google Scholar 

  16. Munthe E, Natvig JB (1972) Complement fixing intracellular complexes of IgG rheumatoid factor in rheumatoid plasma cells. Scand J Immunol 1:217–229

    Google Scholar 

  17. Niebauer GW, Wolf B, Bashey RI, Newton CD (1987) Antibodies to canine collagen types I and II in dogs with spontaneous cruciate ligament rupture and osteoarthritis. Arthritis Rheum 30:319–327

    Google Scholar 

  18. Petersen J, Ingemann-Hansen T, Halkjaer-Kristensen J (1984) Spontaneous and induced immunoglobulin secretion by synovial fluid B lymphocytes in rheumatoid arthritis. Ann Rheum Dis 43:140–145

    Google Scholar 

  19. Pollard KM, Murphy LJ, Webb J (1988) Anti-Sm and anti-DNA antibodies in paired serum and synovial fluid samples from patients with SLE. Rheumatol Int 8:197–204

    Google Scholar 

  20. Rowley MJ, Williamson DJ, Mackay IR (1987) Evidence for local synthesis of antibodies to denatured collagen in the synovium in rheumatoid arthritis. Arthritis Rheum 30:1420–1425

    Google Scholar 

  21. Sabharval UK, Vaughan JH, Fong S (1982) Activation by the classical pathway of complement by rheumatoid factors. Assessment by radioimmunoassay for C4. Arthritis Rheum 25:161–167

    Google Scholar 

  22. Singer II, Kazazis DM, Kawka DW, Rupp EA, Bayne EK (1985) Extracellular matrix interactions in rheumatoid arthritis. Arthritis Rheum 28:1105–1116

    Google Scholar 

  23. Smiley J, Sachs C, Ziff M (1968) In vitro synthesis of immunoglobulin by rheumatoid synovial membrane. J Clin Invest 47:624–632

    Google Scholar 

  24. Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:1271–1277

    Google Scholar 

  25. Van Boxel JA, Paget SA (1975) Predominantly T cell infiltrate in rheumatoid synovial membranes. N Engl J Med 293:517–520

    Google Scholar 

  26. Wood DD, Ihrie EJ, Hamerman D (1985) Release of interleukin-1 from human synovial tissue in vitro. Arthritis Rheum 28:853–861

    Google Scholar 

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Authors and Affiliations

  1. I. Medizinische Klinik, Johannes Gutenberg Universität Mainz, Deutschland

    W. -J. Mayet, E. Hermann, M. Manns & K. -H. Meyer zum Büschenfelde

  2. Institut für Physiologische Chemie, Johannes Gutenberg Universität Mainz, Deutschland

    M. Bachmann

Authors
  1. W. -J. Mayet
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  2. E. Hermann
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  3. M. Bachmann
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  4. M. Manns
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  5. K. -H. Meyer zum Büschenfelde
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Mayet, W.J., Hermann, E., Bachmann, M. et al. Correlation of anti-cytoskeleton antibody activities in synovial fluid with interleukin-6 in patients with osteoarthritis and inflammatory joint disease. Klin Wochenschr 68, 685–691 (1990). https://doi.org/10.1007/BF01667017

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  • DOI: https://doi.org/10.1007/BF01667017

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