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Investigation of the solution structure of hyaluronic acid by light scattering, SAXS, and viscosity measurements

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  • Polymer Science
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Summary

Hyaluronic acid in water exhibits a non-monotonous scattering function, which is explained in terms of multimerization leading to gel-like supermolecular particles. This tendency is highest in water, lower in buffer, and lowest in isotonic NaCl-solution + NaN3. Viscosity measurements show that with increasing concentration a network solution is formed. SAXS-measurements show that nonaggregated Hyaluronic acid is present either in form of double strands, or as supercoils with helical portions.

Zusammenfassung

Hyaluronsäure zeigt in Wasser eine nicht-monotone Streufunktion, die als Ausdruck einer Multimerisation gedeutet wird, welche zu gelartigen übermolekularen Teilchen führt. Diese Tendenz ist in Wasser am stärksten, schwächer in Puffern, und am schwächsten in isotoner NaCl-Lösung + NaN3. Viskositätsmessungen zeigen, daß bei höheren Konzentrationen eine Netzwerk-Lösung gebildet wird. Röntgenkleinwinkel-Messungen ergeben, daß die nicht aggregierte Hyaluronsäure entweder in Form von Doppelsträngen oder als Superknäuel mit helikalen Anteilen vorliegt.

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Authors and Affiliations

  1. Institut für Physikalische, Chemie der Universität Graz, A-8010, Graz, Austria

    G. Ribitsch, J. Schurz & V. Ribitsch

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  1. G. Ribitsch
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  2. J. Schurz
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  3. V. Ribitsch
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Ribitsch, G., Schurz, J. & Ribitsch, V. Investigation of the solution structure of hyaluronic acid by light scattering, SAXS, and viscosity measurements. Colloid & Polymer Sci 258, 1322–1334 (1980). https://doi.org/10.1007/BF01668780

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  • DOI: https://doi.org/10.1007/BF01668780

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