Summary
Mutagenesis ofVicia faba L. using ethyl methanesulfonate has yielded three mutants (f13, f73, f245) with ineffective nodulation (Nod+Fix−), one mutant (f48) unable to nodulate (Nod−) and one supernodulating mutant (f32) having 3 to 5 times more nodules than the control in the absence of combined nitrogen, and expressing nitrate tolerant nodulation (Nod++, Nts). All of the mutant phenotypes were controlled by single and distinct recessive genes designatedsyml tosym5, respectively. Grafting experiments showed that the (Nod+Fix−) and (Nod−) phenotypes were both under root genotype control and that the (Nod++, Nts) phenotype was under shoot genotype control.
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References
Akao, S. & H. Kouchi. 1992. A supernodulating mutant isolated from soybean cultivar Enrei. Soil. Sci. Plant Nutr. 38: 183–187.
Borisov, A.Y., E.V. Morzhina, O.A. Kulikova, S.A. Tchetkova, V.K. Lebsky & I.A. Tikhonovich, 1992. New symbiotic mutants of pea (Pisum sativum L.) affecting either nodule initiation or symbiosome development. Symbiosis 14: 297–313.
Buttery, B.R. & S.J. Park, 1990. Effects of nitrogen inoculation and grafting expression of supernodulation in a mutant ofPhaseolus vulgaris L. Can J. Plant Sci. 70: 375–381.
Buzzel, R.I., B.R. Buttery & G. Ablett, 1992. Super nodulation mutants in Elgin 87 soybean. In: P.M. Gresshoff, L.E. Roth, G. Stacey & W.E. Newton (Eds.), Nitrogen fixation: Achievements and objectives. Routledge, pp. 726. Chapman and Hall Publishers. New York.
Carroll, B.J., D.L. McNeil & P.M. Gresshoff, 1985. A supernodulation and nitrate-tolerant symbiotic (nts) soybean mutant. Plant Physiol 78: 34–40.
Caroll, B.J., D.L. McNeil & P.M. Gresshoff, 1986, Mutagenesis of soybean (Glycine max (L.) Merr.) and the isolation of non-nodulating mutants. Plant Science 47: 109–114.
Davis, T.M., K.E. Giller, J. Kipe-Nolt & M. Awah, 1988. Non nodulating mutants in common bean. Crop Sci. 28: 859–860.
Davis, T.M., K.W. Forster & D.A. Phillips, 1986. Inheritance and expression of three genes controlling root nodule formation in chickpea. Crop Sci. 26: 719–723.
Davis, T.M., 1988. Two genes that confer ineffective nodulation in chickpea (Cicer arietinum L.). J. Heredity 79: 476–478.
Duc, G. & A. Messager, 1989. Mutagenesis of pea (Pisum sativum L.) and the isolation of mutants for nodulation and nitrogen fixation. Plant Science 60: 207–213.
Duc, G. & J. Picard, 1986. Note on the presence of the sym-1 gene inVicia faba hampering its symbiosis withRhizobium leguminosarum Euphytica 35: 61–64.
Engvild, K.C., 1987. Nodulation and nitrogen fixation mutants of pea,Pisum sativum L. Theor. Appl. Genet. 74: 711–713.
Gremaud, M.F. & J.E. Harper, 1989. Selection and initial characterization of partially nitrate tolerant nodulation mutants of soybean. Plant Physiol. 89: 169–173.
Gresshoff, P.M., 1993. Molecular genetic analysis of nodulation genes in soybean. Plant Breeding Reviews 11: 275–318.
Häser, A., D.L. Robinson, C. Duc & C.P. Vance, 1992. A mutation inVicia faba results in ineffective nodules with impaired bacteroïd differentiation and reduced synthesis of late nodulins. J. of Exp. Botany 43: 1397–1407.
Jacobsen, E. & W.J. Feenstra, 1984. A new mutant with efficient nodulation in the presence od nitrate. Plant Sci. lett. 33: 337–344.
Kneen, B.E. & T.A. LaRue, 1984. Nodulation resistant mutant ofPisum sativum L. J. Hered. 75: 238–240.
Kneen, B.E. & T.A. LaRue, 1988. Induced symbiosis mutants of pea (Pisum sativum) and sweetclover (Melilotus alba annua). Plant Science 58: 177–182.
Kneen, B.E., T.A. LaRue, A.M. Hirsch, C.A. Smith & N.F. Weeden, 1990.Sym 13, a gene conditioning ineffective nodulation inPisum sativum. Plant Physiol. 94: 899–905.
LaRue, T.A. & N.F. Weeden, 1992. The symbiosis genes of pea. Pisum Genetics 24: 5–12.
Novak, K., V. Skrdleta, M. Nemcova & M. Vele. 1990. Phenotypic classes of pea nodulation mutants. 5th International Symposium on Molecular Genetics Plant-Microbe Interactions. Interlaken, pp. 392.
Park, S.J. & B.R. Buttery, 1989. Inheritance of nitrate-tolerant supernodulation in EMS-induced mutants of common bean (Phaseolus vulgaris L.). J. Heredity 80: 486–488.
Park, S.J. & B.R. Buttery, 1992. Ethyl-methane sulphonate (EMS) induced nodulation mutants of common bean (Phaseolus vulgaris L.) lacking effective nodules. Plant and Soil 139: 295–298.
Postma, J.G., E. Jacobsen & W.J. Feenstra, 1988. Three pea mutants with an altered nodulation studied by genetic analysis and grafting. J. Plant Physiol. 132: 424–430.
Sagan, M., T. Huguet, D. Barker & G. Duc, 1993a. Characterization of two classes of non-fixing mutants of pea plants (Pisum sativum L.). Plant Science 95: 55–66.
Sagan, M., A. Messager & G. Duc, 1993b. Specifity of theRhizobium-legume symbiosis obtained after mutagenesis in pea (Pisum sativum L.). New Phytol. 125: 757–761.
Sagan, M., T. Huguet & G. Duc, 1994. Phenotypic characterization and classification of nodulation mutants of pea (Pisum sativum L.). Plant Science 100, 59–70.
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Duc, G. Mutagenesis of faba bean (Vicia faba L.) and the identification of five different genes controlling no nodulation, ineffective nodulation or supernodulation. Euphytica 83, 147–152 (1995). https://doi.org/10.1007/BF01678042
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DOI: https://doi.org/10.1007/BF01678042