Skip to main content
SpringerLink
Log in

Differential expression of cell surface integrins on human mast cells and human basophils

  • Original Article
  • Published:
Annals of Hematology Aims and scope Submit manuscript

Summary

Integrins are multifunctional recognition molecules and are expressed on various hematopoietic cells. In the present study expression of integrins on the cell surface of human mast cells and human basophils was investigated by using monoclonal antibodies (mAbs) and indirect immunofluorescence. Human mast cells were obtained from lung (n = 5), uterus (n = 5) and skin (n = 4). Human blood basophils were obtained from normal donors (n = 2). In addition, HMC-1 cells (human mast cell line) and KU-812 cells (a basophil cell line) were analyzed. Primary mast cells were found to react with mAbs directed against the common β chain of β 1 integrins (CD 29), the α chain of VLA-4 (CD49d) and VLA-5 (CD49e), the β chain of β 3 integrins (CD 61), and the α chain of the vitronectin receptor (VNR) (CD 51). Mast cells were not recognized by mAbs to β 2 integrins (CD 18, CD 11 a, CD 11b, CD 11c), the α chain of VLA-2 (CD 49 b), and VLA-6 (CD 49 f). No differences in expression of integrins on human mast cells obtained from different organs were found. HMC-1 cells and primary mast cells expressed an almost identical pattern of integrins. Human basophils and KU-812 cells were found to react with mAbs directed against β 1-integrins (CD 29, CD 49 b, CD 49 d, CD 49 e) and β 2-integrins (CD 18, CD 11 a, CD 11 b, CD 11 c). Together, mast cells and blood basophils express a unique pattern of integrins. These cell surface structures may be involved in the distribution of basophils and tissue mast cells and their accumulation and function in inflammed tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adachi S, Ebi Y, Nishikawa S, Hayashi S, Yamazaki M, Kasugai T, Yamamura T, Nomura S, Kitamura Y (1992) Necessity of extracellular domain of W (c-kit) receptors for attachment of murine cultured mast cells to fibroblasts. Blood 79: 650–656

    Google Scholar 

  2. Bochner BS, Sternbinsky SA (1991) Altered surface expression of CD11 and Leu 8 during human basophil degranulation. J Immunol 146: 2367–2373

    Google Scholar 

  3. Bochner BS, Peachel PT, Brown KE, Schleimer RP (1988) Adherence of human basophils to cultured umbilical vein endothelial cells. J Clin Invest 81: 1355–1364

    Google Scholar 

  4. Bochner BS, MacGlashan DW, Marcotte GV, Schleimer RP (1989) IgE-dependent regulation of human basophil adherence to vascular endothelium. J Immunol 142: 3180–3186

    Google Scholar 

  5. Bochner BS, McKelvey AA, Sterbinsky SA, Hildreth JEK, Derse CP, Klunk DA, Lichtenstein LM, Schleimer RP (1990) Interleukin-3 augments adhesiveness for endothelium and CD 11 b expression in human basophils but not neutrophils. J Immunol 145: 1832–1837

    Google Scholar 

  6. Bochner BS, Luscinskas FW, Gimbrone MA Jr, Newman W, Sterbinsky SA, Derse-Anthony CP, Klunk D, Schleimer RP (1991) Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: contributions of endothelial cell adhesion molecules. J Exp Med 173: 1553–1556

    Google Scholar 

  7. Butterfield JH, Weiler D, Dewald G, Gleich GJ (1988) Establishment of an immature mast cell line from a patient with mast cell leukemia. Leuk Res 12: 345–355

    Google Scholar 

  8. Dastych J, Costa JJ, Thompson HL, Metcalfe DD (1991) Mast cell adhesion to fibronectin. Immunology 73: 478–484

    Google Scholar 

  9. de Boer M, Roos D (1986) Metabolic comparison between basophils and other leukocytes from human blood. J Immunol 136: 3447–3454

    Google Scholar 

  10. Froese A (1984) Receptors for IgE on mast cells and basophils. Prog Allergy 34: 142–187

    Google Scholar 

  11. Galli SJ (1990) Biology of disease: new insights into “the riddle of the mast cell”. Lab Invest 62: 5–33

    Google Scholar 

  12. Guo CB, Sobotka AK, Lichtenstein LM, Bochner BS (1992) Immunophenotyping and functional analysis of purified human uterine mast cells. Blood 79: 708–712

    Google Scholar 

  13. Hemler ME (1990) VLA proteins and the integrin family: structures, functions and their role on leukocytes. Ann Rev Immunol 8: 365–400

    Google Scholar 

  14. Hynes RO (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69: 11–25

    Google Scholar 

  15. Irani AA, Schechter NM, Craig SS, Deblois G, Schwartz LB (1986) Two human mast cell subsets with distinct neutral protease compositions. Proc Natl Acad Sci USA 83: 4064–4068

    Google Scholar 

  16. Ishizaka T, Ishizaka K (1984) Activation of mast cells for mediator release through IgE receptors. Prog Allergy 34: 188–235

    Google Scholar 

  17. Ishizaka T, De Bernardo R, Tomioka H, Lichtenstein LM, Ishizaka K (1972) Identification of the basophil granulocyte as a site of allergic histamine release. J Immunol 108: 1000–1005

    Google Scholar 

  18. Kishi K (1986) A new leukemic cell line with Philadelphia chromosome characterized as basophil precursors. Leuk Res 9: 381–390

    Google Scholar 

  19. Knapp W, Rieber P, Dörken B, Schmidt RE, Stein H, Kr.v.d. Borne AEG (1989) Towards a better definition of human leukocyte surface molecules. Immunol Today 10: 253–258

    Google Scholar 

  20. Lawrence MB, Springer TA (1991) Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 65: 859–873

    Google Scholar 

  21. Lawrence ID, Warner JA, Cohan VL, Hubbard WC, Kagey-Sobotka A, Lichtenstein LM (1987) Purification and characterization of human skin mast cells. J Immunol 139: 3062–3069

    Google Scholar 

  22. Lerner NB, Nocka KH, Cole SR, Qiu F, Strife A, Ashman LK, Besmer P (1991) Monoclonal antibody YB 5.B 8 identifies the human c-kit protein product. Blood 77: 1876–1883

    Google Scholar 

  23. Lichtenstein LM (1971) The immediate allergic response: in vitro separation of antigen activation, decay and histamine release. J Immunol 107: 1122–1127

    Google Scholar 

  24. MacGlashan DW, Schleimer RP, Peters SP, Schulman ES, Adams GK, Sobotka AK, Newball HH, Lichtenstein LM (1983) Comparative studies of human basophils and mast cells. Fed Proc 42: 2504–2509

    Google Scholar 

  25. Massay WA, Guo CB, Dvorak AM, Hrubbard WC, Bhagavan BS, Cohan VL, Warner JA, Sobotka AK, Lichtenstein LM (1991) Human uterine mast cells: purification, characterization, ultrastructure and pharmacology. J Immunol 147: 1621–1627

    Google Scholar 

  26. Mayrhofer G, Gadd SJ, Spargo LDJ, Ashman LK (1987) Specificity of mouse monoclonal antibody raised against acute myeloid leukemia cells in human mucosal and connective tissues. Immunol Cell Biol 65: 241–250

    Google Scholar 

  27. Metzger H, Alcaraz G, Hohman R, Kinet JP, Pribluda V, Quarto R (1986) The receptor with high affinity for immuno-globulin E. Ann Rev Immunol 4: 419–470

    Google Scholar 

  28. Schleimer RP, Sterbinsky SA, Kaiser J, Bickel CA, Klunk DA, Tommioka K, Newman Walter, Luscinskas FW, Gimbrone MA jr, McIntyre BW, Bochner BS (1992) Interleukin-4 induces adherence of human eosinophils and basophils but not neutrophils to endothelium: association with expression of ICAM. J Immunol 148: 1086–1092

    Google Scholar 

  29. Schulman ES, MacGlashan DW, Peters SP, Schleimer RP, Newball HH, Lichtenstein LM (1982) Lung mast cells: purification and characterization. J Immunol 129: 2662–2667

    Google Scholar 

  30. Schwartz LB, Huff TF (1991) Mast cells. In: Crystal RG, West JB, et al. (eds) The lung, pp 601–616

  31. Serafin WE, Austen KF (1987) Mediators of immediate hypersensitivity reactions. N Engl J Med 317: 30–45

    Google Scholar 

  32. Sillaber C, Bevec D, Ashman LK, Butterfield JH, Lechner K, Maurer D, Bettelheim P, Valent P (1991) IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells. J Immunol 147: 4224–4228

    Google Scholar 

  33. Springer TA (1990) Adhesion receptors of the immune system. Nature 346: 425–434

    Google Scholar 

  34. Stain C, Stockinger H, Scharf M, Jäger U, Gössinger H, Lechner K, Bettelheim P (1987) Human blood basophils display a unique phenotype including activation-linked membrane structures. Blood 70: 1872–1878

    Google Scholar 

  35. Thompson HL, Burbelo PD, Metcalfe DD (1990) Regulation of adhesion of mouse bone marrow-derived mast cells to laminin. J Immunol 145: 3425–3431

    Google Scholar 

  36. Thompson HL, Burbelo PD, Gabriel G, Yamada Y, Metcalf DD (1991) Murine mast cells synthesize basement membrane components (a potential role in early fibrosis). J Clin Invest 87: 619–623

    Google Scholar 

  37. Valent P, Ashman LK, Hinterberger W, Eckersberger F, Majdic O, Lechner K, Bettelheim P (1989) Mast cell typing: demonstration of a distinct haemopoietic cell type and evidence for immunophenotypic relationship to mononuclear phagocytes. Blood 73: 1778–1785

    Google Scholar 

  38. Valent P, Majdic O, Maurer D, Bodger M, Muhm M, Bettelheim P (1990) Further characterization of surface membrane structures expressed on human basophils and mast cells. Int Arch Allergy Appl Immunol 91: 198–203

    Google Scholar 

  39. Valent P, Bevec D, Maurer D, Besemer J, di Padova F, Butterfield JH, Speiser W, Majdic O, Lechner K, Bettelheim P (1991) Interleukin-4 promotes expression of mast cell ICAM-1 antigen. Proc Natl Acad Sci USA 88: 3339–3342

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria

    W. R. Sperr, H. Agis, K. Lechner & P. Valent

  2. Department of Gynecology and Obstetrics, University of Vienna, Austria

    K. Czerwenka & E. Kubista

  3. II. Department of Surgery, University of Vienna, Austria

    W. Klepetko

  4. Institute of General and Experimental Pathology, University of Vienna, Austria

    G. Boltz-Nitulescu

Authors
  1. W. R. Sperr
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Agis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Czerwenka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. Klepetko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Kubista
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Boltz-Nitulescu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. Lechner
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. P. Valent
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by theFonds zur Förderung der Wissenschaftlichen Forschung in Österreich, grant no. P-7891

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sperr, W.R., Agis, H., Czerwenka, K. et al. Differential expression of cell surface integrins on human mast cells and human basophils. Ann Hematol 65, 10–16 (1992). https://doi.org/10.1007/BF01715119

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01715119

Key words

Search

Navigation