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Binding sites of atrial natriuretic peptide in human renal tissue — Quantification by in vitro receptor autoradiography

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Summary

Specific binding sites for atrial natriuretic peptide (99–126) in different areas of normal human renal tissue were quantified by in vitro autoradiography. Our data represent the first characterization of ANP binding sites in different structures of the human kidney. Characterization of ANP binding revealed by Scatchard plot analysis a single class of high affinity binding sites in the glomeruli (K d 0.53±0.11 nM;B Max 74.4±17.86 fmol/mg protein), the vasculature (K d 0.18±0.014 nM;B Max 91.6±25.02 fmol/mg protein), and the medulla (K d 0.34±0.13 nM;B Max 106.0±30.61 fmol/mg protein). These sites may play a key role in the actions of the cardiac hormone in human kidney and in the ameliorating effects of ANP in the recovery from acute renal failure.

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Abbreviations

ANP:

Atrial natriuretic peptide

cGMP:

Cyclic guanosine monophosphate

References

  1. Biollaz J, Bidiville J, Diézi J, Waeber B, Nussberger J, Brunner-Ferber F, Gomez HJ, Brunner HR (1987) Site of the action of a synthetic atrial natriuretic peptide evaluated in humans. Kidney Int 32:537–546

    Google Scholar 

  2. Currie MG, Geller MD, Cole BR, Boylan JG, YuSheng W, Holmberg SW, Needleman P (1983) Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria. Science 221:71–73

    Google Scholar 

  3. Eisenhauer T, Talartschik J, Scheler F (1986) Detection of fluid overload by plasma concentration of human atrial natriuretic peptide (h-ANP) in patients with renal failure. Klin Wochenschr [Suppl VI] 64:68–72

    Google Scholar 

  4. Ishikawa Y, Umemura S, Yasuda G, Uchino K, Shindou T, Minamizawa K, Toya Y, Kaneko Y (1987) Identification of an atrial natriuretic peptide specific receptor in human kidney. Biochem Biophys Res Commun 147:135–139

    Google Scholar 

  5. Israel A, Plunkett CM, Saavedra JM (1985) Quantitative autoradiographic characterization of receptors for angiotensin II and other neuropeptides from individual brain nuclei and peripheral tissues from single rats. Cell Mol Neurobiol 5:211–222

    Google Scholar 

  6. Kleinert HD, Maak T, Atlas SA, Januszewicz A, Sealey JE, Laragh JE (1984) Atrial natriuretic factor inhibits angiotensin-, norepinephrine-, and potassium-induced vascular contractility. Hypertension (Suppl I) 6:I-143–I-147

    Google Scholar 

  7. Kuhar MJ (1985) Receptor localization with the microscope In: Yamamura HI, Enna SI, Kuhar MJ (eds) Neurotransmitter receptor binding. Raven Press, New York, pp 255–270

    Google Scholar 

  8. Leitman DC, Andresen JW, Kuno T, Kamisaki Y, Chang JK, Murad F (1986) Identification of multiple binding sites for atrial natriuretic factor by affinity cross-linking in cultured endothelial cells. J Biol Chem 261:11650–11655

    Google Scholar 

  9. Mantyh CR, Kruger L, Brecha NC, Mantyh PW (1986) Localization of specific binding sites for atrial natriuretic factor in peripheral tissues of the guinea pig, rat, and human. Hypertension 8:712–721

    Google Scholar 

  10. Quirion R, Dalpe M, de Léan A, Gutowska J, Cantin M, Genest J (1984) Atrial natriuretic factor (ANF) binding sites in brain and related structures. Peptides 5:1167–1172

    Google Scholar 

  11. Richards AM, Nicholls MG, Ikram H, Webster MW, Yandle TG, Espiner EA (1985) Renal, haemodynamic, and hormonal effects of human alpha atrial natriuretic peptide in healthy volunteers. Lancet I:545–549

    Google Scholar 

  12. Roubert P, Lonchampt MO, Charbier PE, Plas P, Goulin J, Braquet P (1987) Down-regulation of atrial natriuretic factor receptors and correlation with cGMP stimulation in rat cultured vascular smooth muscle cells. Biochem Biophys Res Commun 148:61–67

    Google Scholar 

  13. Schafferhans K, Heidbreder E, Grimm D, Heidland A (1986) Norepinephrine-induced acute renal failure: Beneficial effects of atrial natriuretic factor. Nephron 44:240–244

    Google Scholar 

  14. Sonnenberg H, Honrath U, Chong CK, Wilson DR (1986) Atrial natriuretic factor inhibits sodium transport in medullary collecting duct. Am J Physiol 250:F963-F966

    Google Scholar 

  15. Vlasuk GP, Babilon RW, Nutt RF, Ciccarone TM, Winquist RJ (1987) The actions of atrial natriuretic factor on the vascular wall. Can J Physiol Pharmacol 65:1684–1689

    Google Scholar 

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Authors and Affiliations

  1. Deutsches Primatenzentrum, Göttingen

    A. Brucksch & E. Fuchs

  2. Abteilung für Nephrologie, Universitätsklinik Göttingen, Germany

    J. Talartschik

  3. Abteilung für Pathologie, Universitätsklinik Göttingen, Germany

    H. J. Gröne

Authors
  1. A. Brucksch
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  2. H. J. Gröne
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  3. J. Talartschik
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  4. E. Fuchs
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Brucksch, A., Gröne, H.J., Talartschik, J. et al. Binding sites of atrial natriuretic peptide in human renal tissue — Quantification by in vitro receptor autoradiography. Klin Wochenschr 66, 303–307 (1988). https://doi.org/10.1007/BF01727517

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  • DOI: https://doi.org/10.1007/BF01727517

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