Skip to main content
SpringerLink
Log in

The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells

  • General and Review Articles
  • a. general articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Incubation of HTC cells (7288 C) with 114C-α-linolenic acid in Swim's 77 medium during 24 hours converted the fatty acid to octadeca-6,9,12,15-tetraenoic acid, eicosa-11,14,17-trienoic acid, eicosa-8,11,14,17-tetraenoic acid, eicosa-5,11,14,17-tetraenoic acid, eicosa-5,8,11,14,17-pentaenoic acid and unsaturated acids of 22 carbons. The existence of two pathways was recognized: one initiated by a Δ6-desaturation and the other by an elongation ofα-linolenic acid. Incubation of the cells with insulin and dibutyryl cyclic AMP modified both pathways in different ways. HTC cells were sensitive to insulin which enhanced the de-saturating route increasing eicosapentaenoic acid synthesis and depressed the elongating route decreasing eicosatrienoic acid. In an opposite way, dibutyryl cyclic AMP decreased eicosapentaenoic acid synthesis and increased eicosatrienoic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alaniz, M. J. T. de, Ponz, G. and Brenner, R. R., 1975. Acta Physiol. Latinoamericana, 25, 1–11

    Google Scholar 

  2. Mercuri, O., Peluffo, R. O. and Brenner, R. R., 1967. Lipids 2, 284–285.

    Google Scholar 

  3. Brenner, R. R., Peluffo, R. O., Mercuri, O. and Restelli, M., 1968. Am. J. Physiol. 215, 63–69.

    Google Scholar 

  4. Gómez Dumm, I. N. T. de, Alaniz, M. J. T. de, Brenner, R. R., 1970. J. Lipid Res. 11, 96–101.

    Google Scholar 

  5. Gómez Dumm, I. N. T. de, Alaniz, M. J. T. de and Brenner, R. R., 1975. J. Lipid Res., 16, 264–268.

    Google Scholar 

  6. Thompson, E. B., Tomkins, G. M. and Curran, S. F., 1966. Proc. Natl. Acad. Sci. 56, 296–303.

    Google Scholar 

  7. Goodman, D. S., 1957. Science 125, 1296–1297.

    Google Scholar 

  8. Spector, A. A., Steinberg, D. S. and Tanaka, A., 1965. J. Biol. Chem. 240, 1032–1041.

    Google Scholar 

  9. Castuma, J. C., Catalá, A. and Brenner, R. R., 1972. J. Lipid Res. 13, 783–789.

    Google Scholar 

  10. De Tomás, M. E. and Brenner, R. R., 1964. Ann. Asoc. Quim. Arg. 52, 253–260.

    Google Scholar 

  11. Bray, G. A., 1960. Analytical Biochem. 1, 279–285.

    Google Scholar 

  12. Stein, R. A. and Nicolades, N., 1962. J. Lipid Res. 3, 476–478.

    Google Scholar 

  13. Ullman, D. and Sprecher, H., 1971. Biochim. Biophys. Acta 248, 186–197.

    Google Scholar 

  14. Klenk, E. and Mohrhauer, H., 1960. Z. Physiol. Chem. 320, 218–232.

    Google Scholar 

  15. Brenner, R. R., 1974. Molecular and Cellular Biochemistry 3, 41–52.

    Google Scholar 

  16. Peluffo, R. O., Gómez Dumm, I. N. T. de, Alaniz, M. J. T. de and Brenner, R. R., 1971. J. Nutrition 101, 1075–1084.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina

    María J. T. de Alaniz, I. Nelva T. de Gómez Dumm & Rodolfo R. Brenner

Authors
  1. María J. T. de Alaniz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Nelva T. de Gómez Dumm
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rodolfo R. Brenner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Members of the “Carrera del Investigador” of the “Consejo Nacional de Investigaciones Científicas y Técnicas”, Argentina.

Rights and permissions

Reprints and permissions

About this article

Cite this article

de Alaniz, M.J.T., de Gómez Dumm, I.N.T. & Brenner, R.R. The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells. Mol Cell Biochem 12, 3–8 (1976). https://doi.org/10.1007/BF01731897

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01731897

Keywords

Search

Navigation