Abstract
Quail (Coturnix coturnix japonica) and broiler (Gallus domesticus) chicks were inoculated experimentally with IBH virus (avian adenovirus-1) derived from quails to determine its pathogenicity. Quail chicks were inoculated by the intraperitoneal route at 3, 4, 5, 6 or 7 weeks of age. Lesions were encountered most frequently in the liver, kidneys and lungs. These included pale, swollen and mottled liver, swollen nephrotic kidneys, and congested and pneumonic lungs. The lesions were severe in birds inoculated at 5 weeks of age. Large basophilic intranuclear inclusion bodies were seen in hepatocytes and occasionally in the renal epithelium. The results showed that this isolate is pathogenic for quails above 3 weeks of age. Broiler chicks were inoculated at 4 weeks of age by the intraperitoneal route. The lesions produced in these chicks were similar to those of adenovirus-induced inclusion body hepatitis. Viral antigen was also demonstrated by dot-ELISA in suspensions of liver tissue from both quail and broiler chicks.
Similar content being viewed by others
Abbreviations
- AAF:
-
amnio-allantoic fluid
- AAV:
-
avian adenovirus
- DPI:
-
days post inoculation
- EID50 :
-
dose infective for 50% of embryos
- ELISA:
-
enzyme-linked immunosorbent assay
- IBH:
-
inclusion body hepatitis
- INIBs:
-
intranuclear inclusion bodies
- NAF:
-
normal allantoic fluid
References
Anonymous, 1993.Studies on avian adenoviruses with special reference to epidemiology, pathology and immunology, (Annual report PL-480 project, Punjab Agricultural University, Ludhiana, India)
Grewal, G.S., Singh, A., Singh B. and Oberoi, M.S., 1994. Inclusion body hepatitis in Japanese quail (Coturnix coturnix japonica).Indian Journal of Animal Sciences,64, 665–667
Jack, S.W. and Reed, W.M., 1990. Further characterization of an avian adenovirus associated with inclusion body hepatitis in Bobwhite quails.Avian Diseases,34, 526–530
Jack, S.W., Reed, W.M. and Bryan, T.A., 1987. Inclusion body hepatitis in Bobwhite quail (Colinus virginianus).Avian Diseases,31, 661–665
Khanna, M., Oberoi, M.S., Sawhney, S.M.S. and Sharma, S.N., 1992. Application of dot-enzyme immunoassay for the detection of avian adeno and avian adeno-associated viruses.Indian Journal of Animal Sciences,62, 830–831
Woernle, H., 1966. The use of agar gel diffusion technique in identification of certain avian virus diseases.The Veterinarian,4, 17–28
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singh, A., Oberoi, M.S. & Singh, B. Pathogenicity of quail's inclusion body hepatitis virus (avian adenovirus-1) for Japanese quails and broiler chicks. Vet Res Commun 19, 545–551 (1995). https://doi.org/10.1007/BF01839342
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01839342