Abstract
H-2 antigen variants, derived from a heterozygous mouse Friend leukemia cell line by selection with anti-H-2 antisera and complement, were tested for susceptibility to cell-mediated cytolysis, using T-lymphocytes directed against individual H-2 antigens. The cytotoxic cells were generated in the BALB and B10 backgrounds by a combination of in vivo and in vitro immunizations. The phenotypes of the variants inferred from the patterns of resistance or susceptibility to CML were consistent with those presumed from earlier assays using antisera. The one exception was the variant cell line which was H-2Dd− in assays using antisera, but was H-2Dd+ by CML.
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Bailey, D.W. and Kohn, H.I.: Inherited histocompatibility changes in the progeny of irradiated and unirradiated inbred mice.Genet. Res. 6:330–340, 1965
Cann, H.M. and Herzenberg, L.A.:In vitro studies of mammalian somatic cell variation. II. Isoimmune cytotoxicity with a cultured mouse lymphoma and selection of resistant variants.J. Exp. Med. 117:267–183, 1963
Cerrotini, J.-C., Engers, H.D., Macdonald, H.R., and Brunner, K.T.: Generation of cytotoxic T-lymphocytesin vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures.J. Exp. Med. 140:703–717, 1974
Egorov, I.K.: Genetic control of H-2 alloantigens as inferred from analysis of mutation.Immunogenetics 1:97–107, 1974
Flaherty, L.: TheTla region of the mouse: Identification of a new serologically defined locus, Qa-2.Immunogenetics 2:533–539, 1976
Flaherty, L., Zimmerman, D., and Hansen, T.H.: Further serological analysis of the Qa antigens. Analysis of an anti-H-2.28 serum.Immunogenetics 6:245–251, 1978
Flores, C.F. and Rajan, T.V.: H-2 antigen variants in a cultured mouse leukemia cell line. II. Effect of selection against a K-end gene.Immunogenetics 5:295–308, 1977
Forman, J. and Flaherty, L.: Identification of a new CML target antigen controlled by the Qa-2 locus.Immunogenetics 6:227–233, 1978
Freedman, H.A., and Lilly, F.: Properties of cell lines derived from tumors induced by Friend virus in BALB-H-2b mice.J. Exp. Med. 142:212–223, 1975
Klein, J.: H-2 mutations: Their genetics and effect on immune functions.Adv. Immunol. 26:56–147, 1978
Klein, J. and Chiang, C.: A new locus (H-2T) at theD end of the H-2 complex.Immunogenetics 6:235–243, 1978
McKenzie, I.F.C., Pang, T., and Blanden, R.V.: The use of H-2 mutants as models for the study of T-cell activation.Immunol. Rev. 35:181–230, 1977
Rajan, T.V.: H-2 antigen variants in a cultured heterozygous mouse leukemia cell line.Immunogenetics 4:105–116, 1977
Rajan, T.V. and Flores, C.F.: H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. III. Effect of selection against a D-end gene.Immunogenetics 5:585–596, 1977
Stanton, T.H. and Boyse, E.A.: A new serologically defined locus, Qa-1, in theTla region of the mouse.Immunogenetics 3:525–531, 1976
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Rajan, T.V. H-2 Antigen variants in a cultured heterozygous mouse leukemia cell line. Immunogenetics 7, 457–464 (1978). https://doi.org/10.1007/BF01844035
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DOI: https://doi.org/10.1007/BF01844035