Skip to main content
SpringerLink
Log in

Membrane structural and functional responses to vasopressin in toad bladder

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

Freeze-fracture electronmicroscopy demonstrates that vasopressin stimulation of isolated toad bladder results in a striking morphologic alteration of epithelial membrane structure. This alteration is characterized by the aggregation of intramembranous particles in orderly linear arrays at multiple sites in the luminal membranes of granular cells specifically. The size of these aggregates varies considerably, in terms of area, over a range from 0.5 to 70×10−3 μm2. The median aggregate size is about 10.5×10−3 μm2. Since the extent of vasopressin-associated particle aggregation, in terms of frequency of sites per area of membrane or cumulative area of membrane occupied by them, closely correlates with induced changes in transport function, as measured by osmotic water flow, the aggregates themselves appear to be of physiologic significance in the mechanism of action of vasopressin. This hypothesis is supported by the observations that sites of aggregation occur (a) in response to serosal exposure to hormone specifically, (b) independently of an osmotic gradient, and (c) following stimulation with cyclic adenosine monophosphate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bentley, P.J. 1958. The effects of neurohypophysial extracts on water transfer across the wall of the isolated urinary bladder of the toadBufo marinus. J. Endocrinol.17:201

    Google Scholar 

  2. Bentley, P.J. 1959. The effects of ionic changes on water transfer across the isolated urinary bladder of the toadBufo marinus.J. Endocrinol. 18:327

    Google Scholar 

  3. Bentley, P.J. 1960. The effects of vasopressin on the short-circuit current across the wall of the isolated bladder of the toad,Bufo marinus.J. Endocrinol. 21:161

    Google Scholar 

  4. Branton, D., Bullivant, S., Gilula, N.B., Karnovsky, M.J., Moor, H., Mühlethaler, K., Northcote, D.H., Packer, L., Satir, B., Satir, P., Speth, V., Staehelin, L.A., Steere, R.L., Weinstein, R.S. 1975. Freeze-etching nomenclature.Science 190:54

    Google Scholar 

  5. Chevalier, J., Bourguet, J., Hugon, J.S. 1974. Membrane associated particles: Distribution in frog urinary bladder epithelium at rest and after oxytocin treatment.Cell Tissue Res. 152:129

    Google Scholar 

  6. Civan, M.M., DiBona, D.R. 1974. Pathways for movement of ions and water across toad urinary bladder. II. Site and mode of action of vasopressin.J. Membrane Biol. 19:195

    Google Scholar 

  7. Civan, M.M., Frazier, H.S. 1968. The site of the stimulatory action of vasopressin on sodium transport in toad bladder.J. Gen. Physiol. 51:589

    Google Scholar 

  8. Davis, W.L., Goodman, D.B.P., Martin, J.H., Matthews, J.L., Rasmussen, H. 1974. Vasopressin-induced changes in the toad urinary bladder epithelial surface.J. Cell Biol. 61:544

    Google Scholar 

  9. DeLorenzo, R.J., Greengard, P. 1973. Activation by adenosine 3′∶5′-monophosphate of a membrane-bound phosphoprotein phosphatase from toad bladder.Proc. Nat. Acad. Sci. USA 70:1831

    Google Scholar 

  10. DeLorenzo, R.J., Walton, K.G., Curran, P.F., Greengard, P. 1973. Regulation of phosphorylation of a specific protein in toad-bladder membrane by antidiuretic hormone and cyclic AMP and its possible relationship to membrane permeability changes.Proc. Nat. Acad. Sci. USA 70:880

    Google Scholar 

  11. DiBona, D.R., Civan, M.M., Leaf, A. 1969. The cellular specificity of the effect of vasopressin on toad urinary bladder.J. Membrane Biol. 1:79

    Google Scholar 

  12. Eggena, P. 1973. Inhibition of vasopressin-stimulated urea transport across the toad bladder by thiourea.J. Clin. Invest. 52:2963

    Google Scholar 

  13. Ferguson, D.R., Twite, B.R. 1974. Effects of vasopressin on toad bladder membrane proteins: Relationship to transport of sodium and water.J. Endocrinol. 61:501

    Google Scholar 

  14. Frazier H.S., Dempsey, E.F., Leaf, A. 1962. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin.J. Gen. Physiol. 45:529

    Google Scholar 

  15. Handler, J.S., Butcher, R.W., Sutherland, W., Orloff J. 1965. The effect of vasopressin and of theophylline on the concentration of adenosine 3′,5′-phosphate in the urinary bladder of the toad.J. Biol. Chem. 240:4524

    Google Scholar 

  16. Hays, R.M., Leaf, A. 1962. Studies on the movement of water through the isolated toad bladder and its modification by vasopressin.J. Gen. Physiol. 45:905

    Google Scholar 

  17. Kachadorian, W.A., Levine, S.D., Wade, J.B., Hays, R.M., DiScala, V.A. 1975. Relationship of aggregated intramembranous particles to water permeability in vasopressin (ADH)-treated toad bladder.Kidney Int. 8:481 (Abstr.)

    Google Scholar 

  18. Kachadorian, W.A., Wade, J.B., DiScala, V.A. 1975. Vasopressin: Induced structural change in toad bladder luminal membrane.Science 190:67

    Google Scholar 

  19. Leaf, A. 1960. Some actions of neurophypophyseal hormones on a living membrane.J. Gen. Physiol. 43:175

    Google Scholar 

  20. Levine, S., Franki, N., Hays, R.M. 1973. A saturable, vasopressin-sensitive carrier for urea and acetamide in the toad bladder epithelial cell.J. Clin. Invest. 52:2083

    Google Scholar 

  21. Levine, S., Franki, N., Hays, R.M. 1973. Effect of phloretin on water and solute movement in the toad bladder.J. Clin. Invest. 52:1435

    Google Scholar 

  22. Macknight, A.D.C., Leaf, A., Civan, M.M. 1971. Effects of vasopressin on the water and ionic composition of toad bladder epithelial cells.J. Membrane Biol. 6:127

    Google Scholar 

  23. MacLennan, D.H., Seeman, P., Iles, G.H., Yip, C.C. 1971 Membrane formation by adenosine triphosphatase of sarcoplasmic reticulum.J. Biol. Chem. 246:2702

    Google Scholar 

  24. Maffly, R.H., Hays, R.M., Lamdin, E., Leaf, A. 1960. The effect of neurohypophyseal hormones on the permeability of the toad bladder to urea.J. Clin. Invest. 39:630

    Google Scholar 

  25. McIntyre, J.A., Gilula, N.B., Karnovsky, M.J. 1974. Cryoprotectant-induced redistribution of intramembranous particles in mouse lymphocytes.J. Cell Biol. 60:192

    Google Scholar 

  26. Orloff, J., Handler, J.S. 1962. The similarity of effects of vasopressin, adenosine 3′,5′-phosphate (cyclic AMP) and theophylline on the toad bladder.J. Clin. Invest. 41:702

    Google Scholar 

  27. Peterson, M.J., Edelman, I.S. 1964. Calcium inhibition of the action of vasopressin on the urinary bladder of the toad.J. Clin. Invest. 43:583

    Google Scholar 

  28. Pietras, R.J., Wright, E.M. 1975. The membrane action of antidiuretic hormone (ADH) on toad urinary bladder.J. Membrane Biol. 22:107

    Google Scholar 

  29. Pinto da Silva, P. 1972. Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation.J. Cell Biol. 53:777

    Google Scholar 

  30. Pinto da Silva, P., Douglas, S.D., Branton, D. 1971. Localization of A antigen sites on human erythrocyte ghosts.Nature (London) 232:194

    Google Scholar 

  31. Shuchter, S.H., Franki, N., Hays, R.M. 1973. The effect of tanning agents on the permeability of the toad bladder to water and solutes.J. Membrane Biol. 14:177

    Google Scholar 

  32. Speth, V., Wunderlich, F. 1973. Membranes ofTetrahymena. II. Direct visualization of reversible transitions in biomembrane structure induced by temperature.Biochim. Biophys. Acta 291:621

    Google Scholar 

  33. Spinelli, F., Grosso, A., de Sousa, R.C. 1975. The hydrosmotic effect of vasopressin: A scanning electron-microscope study.J. Membrane Biol. 23:139

    Google Scholar 

  34. Taylor, A., Mamelak, M., Reaven, E., Maffly, R. 1973. Vasopressin: Possible role of microtubules and microfilaments in its action.Science 181:347

    Google Scholar 

  35. Tillack, T.W., Scott, R.E., Marchesi, V.T. 1972. The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles.J. Exp. Med. 135:1209

    Google Scholar 

  36. Tourtellotte, M.E., Zupnik, J.S. 1973. Freeze-fracturedAcholeplasma laidlawii membranes: Nature of particles observed.Science 179:84

    Google Scholar 

  37. Wade, J.B., DiScala, V.A., Karnovsky, M.J. 1975. Membrane structural specialization of the toad bladder revealed by the freeze-fracture technique. I. The granular cell.J. Membrane Biol. 22:385

    Google Scholar 

  38. Walton, K.G., DeLorenzo, R.J., Curran, P.F., Greengard, P. 1975. Regulation of protein phosphorylation and sodium transport in toad bladder.J. Gen. Physiol. 65:153

    Google Scholar 

  39. Yuasa, S., Urakabe, S., Kimura, G., Shirai, D., Takamitsu, Y., Orita, Y., Abe, H. 1975. Effect of colchicine on the osmotic water flow across the toad urinary bladder.Biochim. Biophys. Acta 413:277

    Google Scholar 

Download references

Author information

Author notes

  1. James B. Wade

    Present address: Department of Physiology, Yale University School of Medicine, 06510, New Haven, Connecticut

Authors and Affiliations

  1. Renal Service, U.S. Public Health Service Hospital, 10304, Staten Island, New York

    William A. Kachadorian, James B. Wade, Carole C. Uiterwyk & Vincent A. DiScala

Authors
  1. William A. Kachadorian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James B. Wade
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carole C. Uiterwyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vincent A. DiScala
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kachadorian, W.A., Wade, J.B., Uiterwyk, C.C. et al. Membrane structural and functional responses to vasopressin in toad bladder. J. Membrain Biol. 30, 381–401 (1976). https://doi.org/10.1007/BF01869678

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01869678

Keywords

Search

Navigation