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Dielectric properties of yeast cells

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Summary

Dielectric measurements were made on suspensions of intact yeast cells over a frequency range of 10 kHz to 100 MHz. The suspensions showed typical dielectric dispersions, which are considered to be caused by the presence of cytoplasmic membranes with sufficiently low conductivity. Since the conductivity of the cell wall was found to be of nearly the same value as that of the suspending medium, composed of KCl solutions in a range from 10 to 80mm, the cell wall may be ignored in establishing an electrical model of the cells suspended in such media. An analysis of the dielectric data was carried out by use of Pauly and Schwan's theory. The membrane capacitance was estimated to be 1.1±0.1 μF/cm2, which is compared with values reported so far for most biological membranes. The conductivity of the cell interior was almost unchanged with varying KCl concentrations and showed low values owing to the presence of less conducting particles, presumably intracellular organelles. The relatively low dielectric constant of about 50 obtained for the cell interior, in comparison with values of aqueous solutions, may be attributed also to the presence of intracellular organelles and proteins.

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Authors and Affiliations

  1. Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan

    Koji Asami, Tetsuya Hanai & Naokazu Koizumi

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  1. Koji Asami
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  2. Tetsuya Hanai
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  3. Naokazu Koizumi
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Asami, K., Hanai, T. & Koizumi, N. Dielectric properties of yeast cells. J. Membrain Biol. 28, 169–180 (1976). https://doi.org/10.1007/BF01869695

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  • DOI: https://doi.org/10.1007/BF01869695

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