Summary
Ethidium bromide and other cationic dyes have been found to inhibit movalent cation uptake. This dye also produces in a K+-free medium an efflux of K+ which could be of the electrogenic type.
The study of the effects of the same cationic dyes on Ca2+ uptake showed a large stimulation of the uptake rate of the divalent cation of more than tenfold.
The analysis of the effects of one of the cationic dyes on Ca2+ uptake indicated that the efflux of K+ is of the electrogenic type and can drive the uptake of the divalent cation.
Kinetic data on Ca2+ uptake indicate that, both under “normal” or under stimulated conditions, the divalent cation is taken up by the same transport system.
The addition of ethidium bromide, besides, can stimulate the uptake of Mn2+ and14C-glycine and could be a good weapon to magnify and study some of the characteristics of ion transport systems in yeast.
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Peña, A. Effect of ethidium bromide on Ca2+ uptake by yeast. J. Membrain Biol. 42, 199–213 (1978). https://doi.org/10.1007/BF01870358
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DOI: https://doi.org/10.1007/BF01870358