Summary
A model has been developed for 5-nitroxide stearate, I(12,3), distribution in human erythrocyte ghosts which accurately predicts ESR spectral alterations observed with increased probe/total lipid (P/L) at 37°C. This spin probe occupies a class of high-affinity, noninteracting sites at low loading. Saturation occurs with increasing probe concentration, and, at higher loading, the probe inserts itself at initially dilute sites to form membranebound clusters of variable size. No ‘low’ probe remains at high P/L where all I(12,3) clusters in a ‘concentrated’ phase. This model allows determination of the dilute/clustered probe ratio, and shows that I(12,3) segregates in erythrocytes at what might otherwise be considered low P/L (e.g., 1/359). These findings validate the earlier use of empirical parameters to estimate probe sequestration in biological membranes.
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Gordon, L.M., Looney, F.D. & Curtain, C.C. Spin probe clustering in human erythrocyte ghosts. J. Membrain Biol. 84, 81–95 (1985). https://doi.org/10.1007/BF01871650
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DOI: https://doi.org/10.1007/BF01871650