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Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata)

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Abstract

The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high α-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.

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Authors and Affiliations

  1. Departmento de Biología Aplicada, Escuela Politécnica Superior, Universidad de Almería, 04120, Almería, Spain

    F. J. Moyano, M. Díaz & F. J. Alarcón

  2. Instituto Ciencias Marinas Andalucía, Poligono Río San Pedro, Puerto Real, Cádiz, Spain

    M. C. Sarasquete

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  1. F. J. Moyano
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  2. M. Díaz
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  3. F. J. Alarcón
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  4. M. C. Sarasquete
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Moyano, F.J., Díaz, M., Alarcón, F.J. et al. Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata). Fish Physiol Biochem 15, 121–130 (1996). https://doi.org/10.1007/BF01875591

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  • DOI: https://doi.org/10.1007/BF01875591

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